Lineage Dependence of Cell Chirality in Human Mesenchymal Stem Cell

細胞手性於人類間充質幹細胞的譜系從屬性

Student thesis: Doctoral Thesis

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Award date8 Jan 2019

Abstract

Cellular mechanical behaviors have been known as an essential factor for cell functions, including migration, gene expression and differentiation. Investigation on the correlation between cell mechanics and differentiation have been widely conducted. Recently, as one of important mechanical behaviors, cellular chirality has been observed and demonstrated as an intrinsic property depending on cell types. However, how the traditional chemical-based differentiation process mediates the mechanotransduction and biochemical processes toward cell chiral behavior remains largely unclear. Here, using cell patterning and time-lapse microscopy, we have successfully revealed the chiral orientation/rotation behaviors of human mesenchymal stem cells (hMSCs). Furthermore, by examining the cellular orientation and rotation among hMSCs and induced adipocytes/osteoblasts, we found the cell chirality exhibited a lineage dependence, and a reconfiguration of swirling actin structure with lineage dependence may underlie this chiral response. More importantly, the rotation and actin configuration appeared at early stage of differentiation, suggesting that early established chirality may serve as the framework for stem cell linage commitment. Together, this work reveals the existence of hMSCs chirality and its linage dependence. The chiral response at early stage of chemical induction further demonstrates the participation of cytoskeleton in cell differentiation. Providing a role of cell chirality in differentiation process, our results pave the way of understanding mechanotransduction in cell differentiation in the future.