Engineering Extracellular Vesicle for Efficient Delivery of Therapeutic Cargo

合成外囊泡為高效藥物載體

Student thesis: Doctoral Thesis

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Award date13 Aug 2021

Abstract

Extracellular vesicles (EVs) have gained much attention due to their function as a means of intercellular communication. A long- distance transfer of RNA molecules bolsters the potential use of EVs as a natural carrier for genetic materials to target cells. However, there are several hurdles preventing extracellular vesicles from being ideal and effective carriers. By engineering the extracellular vesicles with functional molecules, the equipped vesicles could tackle the barriers which thwarting them from deliver therapeutic cargo into target cells. In the first chapter of this dissertation, I will discuss the advantages of extracellular vesicles and the current limit in harnessing their natural carrier property for therapeutic use. The second chapter addresses the targeting delivery and describes an enzymatic method to conjugate targeting molecules to the surface of red blood cell EVs (RBCEVs) for targeted delivery. Chapter three presents a deeper understanding of uptake, intracellular trafficking and cargo release of RBCEVs in Hela cells. Finally, we will discuss the advantages, and limitations of utilizing RBCEVs together with their future perspectives in targeted delivery.