Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate

Jiangbo Chen; Yachao Zhang; Linyun He; Yizhi Liang; Lidai Wang

doi:10.1016/j.pacs.2020.100195

Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

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Author(s)

Jiangbo Chen
Yachao Zhang
Linyun He
Yizhi Liang
Lidai Wang

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Detail(s)

Original language	English
Article number	100195
Journal / Publication	Photoacoustics
Volume	20
Online published	7 Jun 2020
Publication status	Published - Dec 2020

Link(s)

DOI	DOI https://doi.org/10.1016/j.pacs.2020.100195 Final Published version
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Attachment(s)	Documents 53847906.pdf Final Published version, 1.86 MB, PDF Publisher's Copyright Statement This full text is made available under CC-BY-NC-ND 4.0. https://creativecommons.org/licenses/by-nc-nd/4.0/
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-85086504181&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(8a8bf059-17fd-4d5d-b60c-6c86179a3ca3).html

Abstract

We report wide-field polygon-scanning functional OR-PAM that for the first time achieves 1-MHz A-line rate of oxygen saturation in vivo. We address two technical challenges. The first is a 1-MHz dual-wavelength pulsed laser that has sufficient pulse energy and ultrafast wavelength switching. The second is a polygon-scanning imaging probe that has a fast scanning speed, a large field of view, and great sensitivity. The OR-PAM system offers a B-scan rate of 477.5 Hz in a 12-mm range and a volumetric imaging rate of ∼1 Hz over a 12 × 5 mm² scanning area. We image microvasculature and blood oxygen saturation in a 12 × 12 mm² scanning area in 5 s. Dynamic imaging of oxygen saturation in the mouse ear is demonstrated to monitor fast response to epinephrine injection. The new wide-field fast functional imaging ability broadens the biomedical application of OR-PAM.