Wide-field photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with host publication)peer-review

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Author(s)

Related Research Unit(s)

Detail(s)

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2021
EditorsAlexander A. Oraevsky, Lihong V. Wang
PublisherSPIE
ISBN (Electronic) 9781510641204
ISBN (Print)9781510641198
Publication statusPublished - 2021

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11642
ISSN (Print)1605-7422

Conference

TitleSPIE BiOS 2021 Digital Forum
LocationOnline
PlaceUnited States
CityCalifornia
Period6 - 11 March 2021

Abstract

We report wide-field polygon-scanning optical-resolution photoacoustic microscopy (OR-PAM) that achieves 1-MHz A-line rate of oxygen saturation in vivo. We develop a polygon-scanning imaging probe. Using stimulated Raman scattering and optical delay in fiber, we develop a dual-wavelength pulsed laser that has proper wavelengths, sufficient pulse energy, high pulse repetition rate, and sub-microseconds wavelength switching time for fast imaging of oxygen saturation. The OR-PAM system offers a 478-Hz B-scan rate and a 1-Hz volumetric imaging rate over a 12×5 mm2 scanning area. We demonstrate dynamic imaging of oxygen saturation of fast response to epinephrine injection in the mouse ear. Results show fast hemodynamic and functional changes in centimeter-sized areas.

Research Area(s)

  • fast imaging, functional imaging, oxygen saturation, Photoacoustic microscopy, wide-field

Citation Format(s)

Wide-field photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate. / Chen, Jiangbo; Zhang, Yachao; He, Linyun et al.
Photons Plus Ultrasound: Imaging and Sensing 2021. ed. / Alexander A. Oraevsky; Lihong V. Wang. SPIE, 2021. 116422U (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11642).

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with host publication)peer-review