Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Junjie Yao
  • Chih-Hsien Huang
  • Joon-Mo Yang
  • Liang Gao
  • Konstantin I. Maslov
  • Jun Zou
  • Lihong V. Wang

Detail(s)

Original languageEnglish
Article number80505
Journal / PublicationJournal of Biomedical Optics
Volume17
Issue number8
Publication statusPublished - Aug 2012
Externally publishedYes

Abstract

By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have to sacrifice either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning ORPAM by using a water-immersible microelectromechanical systems (MEMS) scanning mirror (MEMS-OR-PAM). In MEMS-OR-PAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures the uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. Presented results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena. © 2012 Society of Photo-Optical Instrumentation Engineers (SPIE).

Research Area(s)

  • Blood flow dynamics, Confocal sensitivity, Confocality, Fast scanning, MEMS scanning mirror, Optical-resolution photoacoustic microscopy

Citation Format(s)

Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror. / Yao, Junjie; Huang, Chih-Hsien; Wang, Lidai et al.
In: Journal of Biomedical Optics, Vol. 17, No. 8, 80505, 08.2012.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review