Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate

Jiangbo Chen; Yachao Zhang; Linyun He; Yizhi Liang; Lidai Wang

doi:10.1016/j.pacs.2020.100195

Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate

Jiangbo Chen
, Yachao Zhang
, Linyun He
, Yizhi Liang^*
, Lidai Wang^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

96 Citations (Scopus)

102 Downloads (CityUHK Scholars)

Abstract

We report wide-field polygon-scanning functional OR-PAM that for the first time achieves 1-MHz A-line rate of oxygen saturation in vivo. We address two technical challenges. The first is a 1-MHz dual-wavelength pulsed laser that has sufficient pulse energy and ultrafast wavelength switching. The second is a polygon-scanning imaging probe that has a fast scanning speed, a large field of view, and great sensitivity. The OR-PAM system offers a B-scan rate of 477.5 Hz in a 12-mm range and a volumetric imaging rate of ∼1 Hz over a 12 × 5 mm² scanning area. We image microvasculature and blood oxygen saturation in a 12 × 12 mm² scanning area in 5 s. Dynamic imaging of oxygen saturation in the mouse ear is demonstrated to monitor fast response to epinephrine injection. The new wide-field fast functional imaging ability broadens the biomedical application of OR-PAM.

Original language	English
Article number	100195
Journal	Photoacoustics
Volume	20
Online published	7 Jun 2020
DOIs	https://doi.org/10.1016/j.pacs.2020.100195
Publication status	Published - Dec 2020

Research Keywords

Functional imaging
Oxygen saturation
Photoacoustic microscopy
Wide-Field

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This full text is made available under CC-BY-NC-ND 4.0. https://creativecommons.org/licenses/by-nc-nd/4.0/

RGC Funding Information

RGC-funded

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10.1016/j.pacs.2020.100195

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title = "Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate",

abstract = "We report wide-field polygon-scanning functional OR-PAM that for the first time achieves 1-MHz A-line rate of oxygen saturation in vivo. We address two technical challenges. The first is a 1-MHz dual-wavelength pulsed laser that has sufficient pulse energy and ultrafast wavelength switching. The second is a polygon-scanning imaging probe that has a fast scanning speed, a large field of view, and great sensitivity. The OR-PAM system offers a B-scan rate of 477.5 Hz in a 12-mm range and a volumetric imaging rate of ∼1 Hz over a 12 × 5 mm2 scanning area. We image microvasculature and blood oxygen saturation in a 12 × 12 mm2 scanning area in 5 s. Dynamic imaging of oxygen saturation in the mouse ear is demonstrated to monitor fast response to epinephrine injection. The new wide-field fast functional imaging ability broadens the biomedical application of OR-PAM.",

keywords = "Functional imaging, Oxygen saturation, Photoacoustic microscopy, Wide-Field, Functional imaging, Oxygen saturation, Photoacoustic microscopy, Wide-Field, Functional imaging, Oxygen saturation, Photoacoustic microscopy, Wide-Field",

author = "Jiangbo Chen and Yachao Zhang and Linyun He and Yizhi Liang and Lidai Wang",

year = "2020",

month = dec,

doi = "10.1016/j.pacs.2020.100195",

language = "English",

volume = "20",

journal = "Photoacoustics",

issn = "2213-5979",

publisher = "Elsevier GmbH",

}

TY - JOUR

T1 - Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate

AU - Chen, Jiangbo

AU - Zhang, Yachao

AU - He, Linyun

AU - Liang, Yizhi

AU - Wang, Lidai

PY - 2020/12

Y1 - 2020/12

N2 - We report wide-field polygon-scanning functional OR-PAM that for the first time achieves 1-MHz A-line rate of oxygen saturation in vivo. We address two technical challenges. The first is a 1-MHz dual-wavelength pulsed laser that has sufficient pulse energy and ultrafast wavelength switching. The second is a polygon-scanning imaging probe that has a fast scanning speed, a large field of view, and great sensitivity. The OR-PAM system offers a B-scan rate of 477.5 Hz in a 12-mm range and a volumetric imaging rate of ∼1 Hz over a 12 × 5 mm2 scanning area. We image microvasculature and blood oxygen saturation in a 12 × 12 mm2 scanning area in 5 s. Dynamic imaging of oxygen saturation in the mouse ear is demonstrated to monitor fast response to epinephrine injection. The new wide-field fast functional imaging ability broadens the biomedical application of OR-PAM.

AB - We report wide-field polygon-scanning functional OR-PAM that for the first time achieves 1-MHz A-line rate of oxygen saturation in vivo. We address two technical challenges. The first is a 1-MHz dual-wavelength pulsed laser that has sufficient pulse energy and ultrafast wavelength switching. The second is a polygon-scanning imaging probe that has a fast scanning speed, a large field of view, and great sensitivity. The OR-PAM system offers a B-scan rate of 477.5 Hz in a 12-mm range and a volumetric imaging rate of ∼1 Hz over a 12 × 5 mm2 scanning area. We image microvasculature and blood oxygen saturation in a 12 × 12 mm2 scanning area in 5 s. Dynamic imaging of oxygen saturation in the mouse ear is demonstrated to monitor fast response to epinephrine injection. The new wide-field fast functional imaging ability broadens the biomedical application of OR-PAM.

KW - Functional imaging

KW - Oxygen saturation

KW - Photoacoustic microscopy

KW - Wide-Field

KW - Functional imaging

KW - Oxygen saturation

KW - Photoacoustic microscopy

KW - Wide-Field

KW - Functional imaging

KW - Oxygen saturation

KW - Photoacoustic microscopy

KW - Wide-Field

UR - https://www.scopus.com/pages/publications/85086504181

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85086504181&origin=recordpage

U2 - 10.1016/j.pacs.2020.100195

DO - 10.1016/j.pacs.2020.100195

M3 - RGC 21 - Publication in refereed journal

C2 - 32577378

SN - 2213-5979

VL - 20

JO - Photoacoustics

JF - Photoacoustics

M1 - 100195

ER -

Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate

Abstract

Research Keywords

Publisher's Copyright Statement

RGC Funding Information

Access Output

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GRF: Development of Optical-resolution Photoacoustic Microscopy with Compensation of Absorption Saturation

GRF: Quantitative Photoacoustic Imaging of Blood Oxygen Saturation in Deep Tissue

ECS: Large-Scale Single-Cell Photoacoustic Microscopy of the Brain in Action

Ultrasound/ Photoacoustic Imaging Platform, Tumor Treatment System Development and Their Biomedical Applications

Cite this

Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate

Abstract

Research Keywords

Publisher's Copyright Statement

RGC Funding Information

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Projects

GRF: Development of Optical-resolution Photoacoustic Microscopy with Compensation of Absorption Saturation

GRF: Quantitative Photoacoustic Imaging of Blood Oxygen Saturation in Deep Tissue

ECS: Large-Scale Single-Cell Photoacoustic Microscopy of the Brain in Action

Student theses

Ultrasound/ Photoacoustic Imaging Platform, Tumor Treatment System Development and Their Biomedical Applications

Cite this