Wide-field photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate

Jiangbo Chen, Yachao Zhang, Linyun He, Yizhi Liang, Lidai Wang

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review

Abstract

We report wide-field polygon-scanning optical-resolution photoacoustic microscopy (OR-PAM) that achieves 1-MHz A-line rate of oxygen saturation in vivo. We develop a polygon-scanning imaging probe. Using stimulated Raman scattering and optical delay in fiber, we develop a dual-wavelength pulsed laser that has proper wavelengths, sufficient pulse energy, high pulse repetition rate, and sub-microseconds wavelength switching time for fast imaging of oxygen saturation. The OR-PAM system offers a 478-Hz B-scan rate and a 1-Hz volumetric imaging rate over a 12×5 mm2 scanning area. We demonstrate dynamic imaging of oxygen saturation of fast response to epinephrine injection in the mouse ear. Results show fast hemodynamic and functional changes in centimeter-sized areas.
Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2021
EditorsAlexander A. Oraevsky, Lihong V. Wang
PublisherSPIE
ISBN (Electronic) 9781510641204
ISBN (Print)9781510641198
DOIs
Publication statusPublished - 2021
EventSPIE BiOS 2021 Digital Forum - Online, California, United States
Duration: 6 Mar 202111 Mar 2021

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11642
ISSN (Print)1605-7422

Conference

ConferenceSPIE BiOS 2021 Digital Forum
PlaceUnited States
CityCalifornia
Period6/03/2111/03/21

Research Keywords

  • fast imaging
  • functional imaging
  • oxygen saturation
  • Photoacoustic microscopy
  • wide-field

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