WHOLE TUMOR SCREENING WITH SINGLE CELL MULTIPLEXED PROTEOLYTIC ASSAY THROUGH CONTINUOUS FLOW MICROFLUIDICS

Ee Xien Ng; Guoyun Sun; Shih-Chung Wei; Miles A. Miller; Chia-Hung Chen

WHOLE TUMOR SCREENING WITH SINGLE CELL MULTIPLEXED PROTEOLYTIC ASSAY THROUGH CONTINUOUS FLOW MICROFLUIDICS

Ee Xien Ng
, Guoyun Sun
, Shih-Chung Wei
, Miles A. Miller
, Chia-Hung Chen^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

A droplet-based high throughput platform was developed to screen single cell proteolytic enzymatic activity in a continuous flow manner, as a functional assay based flow cytometry (Droplet-FACS). With the capability to screen ~50 cells per second, whole primary tumor screening (~2x10⁵ cells) was approached within 60 minutes to allow rapid profiling of patients' tumors based single cell multiplexed enzymatic assay. Moreover, the aggressive sub group of cancer cells could be identified and sorted for down-stream gene analysis.

Original language	English
Title of host publication	μTAS 2017
Subtitle of host publication	The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	139-140
ISBN (Electronic)	9780692941836
Publication status	Published - Oct 2017
Externally published	Yes
Event	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017) - Savannah, United States Duration: 22 Oct 2017 → 26 Oct 2017 https://archive.cbmsociety.org/utas/uTAS2017.zip

Publication series

Name	International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS
Publisher	Chemical and Biological Microsystems Society
ISSN (Electronic)	1556-5904

Conference

Conference	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)
Place	United States
City	Savannah
Period	22/10/17 → 26/10/17
Internet address	https://archive.cbmsociety.org/utas/uTAS2017.zip

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

Proteolytic activity assay
Single cell analysis
Whole tumor analysis

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Ng, E. X., Sun, G., Wei, S.-C., Miller, M. A., & Chen, C.-H. (2017). WHOLE TUMOR SCREENING WITH SINGLE CELL MULTIPLEXED PROTEOLYTIC ASSAY THROUGH CONTINUOUS FLOW MICROFLUIDICS. In μTAS 2017: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 139-140). (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS). Chemical and Biological Microsystems Society.

Ng, Ee Xien ; Sun, Guoyun ; Wei, Shih-Chung et al. / WHOLE TUMOR SCREENING WITH SINGLE CELL MULTIPLEXED PROTEOLYTIC ASSAY THROUGH CONTINUOUS FLOW MICROFLUIDICS. μTAS 2017: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2017. pp. 139-140 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

@inproceedings{16d4f26538d14ef08a23e2cac8cf548a,

title = "WHOLE TUMOR SCREENING WITH SINGLE CELL MULTIPLEXED PROTEOLYTIC ASSAY THROUGH CONTINUOUS FLOW MICROFLUIDICS",

abstract = "A droplet-based high throughput platform was developed to screen single cell proteolytic enzymatic activity in a continuous flow manner, as a functional assay based flow cytometry (Droplet-FACS). With the capability to screen \textasciitilde{}50 cells per second, whole primary tumor screening (\textasciitilde{}2x105 cells) was approached within 60 minutes to allow rapid profiling of patients' tumors based single cell multiplexed enzymatic assay. Moreover, the aggressive sub group of cancer cells could be identified and sorted for down-stream gene analysis.",

keywords = "Proteolytic activity assay, Single cell analysis, Whole tumor analysis",

author = "Ng, \{Ee Xien\} and Guoyun Sun and Shih-Chung Wei and Miller, \{Miles A.\} and Chia-Hung Chen",

year = "2017",

month = oct,

language = "English",

series = "International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS",

publisher = "Chemical and Biological Microsystems Society",

pages = "139--140",

booktitle = "μTAS 2017",

note = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017) ; Conference date: 22-10-2017 Through 26-10-2017",

url = "https://archive.cbmsociety.org/utas/uTAS2017.zip",

}

Ng, EX, Sun, G, Wei, S-C, Miller, MA & Chen, C-H 2017, WHOLE TUMOR SCREENING WITH SINGLE CELL MULTIPLEXED PROTEOLYTIC ASSAY THROUGH CONTINUOUS FLOW MICROFLUIDICS. in μTAS 2017: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS, Chemical and Biological Microsystems Society, pp. 139-140, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017), Savannah, Georgia, United States, 22/10/17.

WHOLE TUMOR SCREENING WITH SINGLE CELL MULTIPLEXED PROTEOLYTIC ASSAY THROUGH CONTINUOUS FLOW MICROFLUIDICS. / Ng, Ee Xien; Sun, Guoyun; Wei, Shih-Chung et al.
μTAS 2017: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2017. p. 139-140 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - WHOLE TUMOR SCREENING WITH SINGLE CELL MULTIPLEXED PROTEOLYTIC ASSAY THROUGH CONTINUOUS FLOW MICROFLUIDICS

AU - Ng, Ee Xien

AU - Sun, Guoyun

AU - Wei, Shih-Chung

AU - Miller, Miles A.

AU - Chen, Chia-Hung

PY - 2017/10

Y1 - 2017/10

N2 - A droplet-based high throughput platform was developed to screen single cell proteolytic enzymatic activity in a continuous flow manner, as a functional assay based flow cytometry (Droplet-FACS). With the capability to screen ~50 cells per second, whole primary tumor screening (~2x105 cells) was approached within 60 minutes to allow rapid profiling of patients' tumors based single cell multiplexed enzymatic assay. Moreover, the aggressive sub group of cancer cells could be identified and sorted for down-stream gene analysis.

AB - A droplet-based high throughput platform was developed to screen single cell proteolytic enzymatic activity in a continuous flow manner, as a functional assay based flow cytometry (Droplet-FACS). With the capability to screen ~50 cells per second, whole primary tumor screening (~2x105 cells) was approached within 60 minutes to allow rapid profiling of patients' tumors based single cell multiplexed enzymatic assay. Moreover, the aggressive sub group of cancer cells could be identified and sorted for down-stream gene analysis.

KW - Proteolytic activity assay

KW - Single cell analysis

KW - Whole tumor analysis

UR - https://www.scopus.com/pages/publications/85079682081

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85079682081&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

T3 - International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS

SP - 139

EP - 140

BT - μTAS 2017

PB - Chemical and Biological Microsystems Society

T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)

Y2 - 22 October 2017 through 26 October 2017

ER -

Ng EX, Sun G, Wei SC, Miller MA, Chen CH. WHOLE TUMOR SCREENING WITH SINGLE CELL MULTIPLEXED PROTEOLYTIC ASSAY THROUGH CONTINUOUS FLOW MICROFLUIDICS. In μTAS 2017: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2017. p. 139-140. (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

WHOLE TUMOR SCREENING WITH SINGLE CELL MULTIPLEXED PROTEOLYTIC ASSAY THROUGH CONTINUOUS FLOW MICROFLUIDICS

Abstract

Publication series

Conference

UN SDGs

Research Keywords

Other Access Options

Permanent Link

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