Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review

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Author(s)

  • Junjie Yao
  • Chih-Hsien Huang
  • Catherine Martel
  • Konstantin I. Maslov
  • Joon-Mo Yang
  • Liang Gao
  • Gwendalyn Randolph
  • Jun Zou
  • Lihong V. Wang

Detail(s)

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8581
Publication statusPublished - 2013
Externally publishedYes

Publication series

Name
Volume8581
ISSN (Print)1605-7422

Conference

TitlePhotons Plus Ultrasound: Imaging and Sensing 2013
PlaceUnited States
CitySan Francisco, CA
Period3 - 5 February 2013

Abstract

By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have sacrificed either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible MEMS scanning mirror (MEMS-ORPAM). Made of silicon with a gold coating, the MEMS mirror plate can reflect both optical and acoustic beams. Because it uses an electromagnetic driving force, the whole MEMS scanning system can be submerged in water. In MEMS-ORPAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. A diffraction-limited lateral resolution of 2.4 μm in water and a maximum imaging depth of 1.1 mm in soft tissue have been experimentally determined. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. By using Evans blue dye as the contrast agent, we also imaged the flow dynamics of lymphatic vessels in a mouse tail in vivo. The results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena. © 2013 Copyright SPIE.

Research Area(s)

  • blood flow dynamic imaging, functional brain imaging, lymphatic flow dynamic imaging, MEMS scanning mirror, Optical-resolution photoacoustic microscopy

Citation Format(s)

Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy. / Yao, Junjie; Huang, Chih-Hsien; Martel, Catherine et al.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8581 2013. 858127.

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review