TY - GEN
T1 - Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy
AU - Yao, Junjie
AU - Huang, Chih-Hsien
AU - Martel, Catherine
AU - Maslov, Konstantin I.
AU - Wang, Lidai
AU - Yang, Joon-Mo
AU - Gao, Liang
AU - Randolph, Gwendalyn
AU - Zou, Jun
AU - Wang, Lihong V.
PY - 2013
Y1 - 2013
N2 - By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have sacrificed either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible MEMS scanning mirror (MEMS-ORPAM). Made of silicon with a gold coating, the MEMS mirror plate can reflect both optical and acoustic beams. Because it uses an electromagnetic driving force, the whole MEMS scanning system can be submerged in water. In MEMS-ORPAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. A diffraction-limited lateral resolution of 2.4 μm in water and a maximum imaging depth of 1.1 mm in soft tissue have been experimentally determined. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. By using Evans blue dye as the contrast agent, we also imaged the flow dynamics of lymphatic vessels in a mouse tail in vivo. The results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena. © 2013 Copyright SPIE.
AB - By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have sacrificed either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible MEMS scanning mirror (MEMS-ORPAM). Made of silicon with a gold coating, the MEMS mirror plate can reflect both optical and acoustic beams. Because it uses an electromagnetic driving force, the whole MEMS scanning system can be submerged in water. In MEMS-ORPAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. A diffraction-limited lateral resolution of 2.4 μm in water and a maximum imaging depth of 1.1 mm in soft tissue have been experimentally determined. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. By using Evans blue dye as the contrast agent, we also imaged the flow dynamics of lymphatic vessels in a mouse tail in vivo. The results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena. © 2013 Copyright SPIE.
KW - blood flow dynamic imaging
KW - functional brain imaging
KW - lymphatic flow dynamic imaging
KW - MEMS scanning mirror
KW - Optical-resolution photoacoustic microscopy
UR - https://www.scopus.com/pages/publications/84878082257
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84878082257&origin=recordpage
U2 - 10.1117/12.2005669
DO - 10.1117/12.2005669
M3 - RGC 32 - Refereed conference paper (with host publication)
SN - 9780819493507
VL - 8581
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
T2 - Photons Plus Ultrasound: Imaging and Sensing 2013
Y2 - 3 February 2013 through 5 February 2013
ER -