Vascular lumen simulation and highly-sensitive nitric oxide detection using three-dimensional gelatin chip coupled to TiC/C nanowire arrays microelectrode
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Detail(s)
Original language | English |
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Pages (from-to) | 4249-4256 |
Journal / Publication | Lab on a Chip - Miniaturisation for Chemistry and Biology |
Volume | 12 |
Issue number | 21 |
Publication status | Published - 7 Nov 2012 |
Link(s)
Abstract
Reproducing the physiological environment of blood vessels for the in vitro investigation of endothelial cell functions is very challenging. Here, we describe a vascular-like structure based on a three-dimensional (3D) gelatin chip with good compatibility and permeability which is also cost-effective and easy to produce. The controllable lumen diameter and wall thickness enable close mimicking of blood vessels in vitro. The 3D gelatin matrix between adjacent lumens is capable of generating soluble-factor gradients inside, and diffusion of molecules with different molecular weights through the matrix is studied. The cultured human umbilical vein endothelial cells proliferate on the gelatin lumen linings to form a vascular lumen. The hemodynamic behavior including adhesion, alignment of endothelial cells (ECs) under shear stress and pulsatile stretch is studied. Furthermore, a microelectrode comprising TiC/C nanowire arrays is fabricated to detect nitric oxide with sub-nM detection limits and NO generation from the cultured ECs is monitored in real time. This vascular model reproduces the surrounding parenchyma of endothelial cells and mimics the hemodynamics inside blood vessels very well, thereby enabling potential direct investigation of hemodynamics, angiogenesis, and tumor metastasis in vitro. © 2012 The Royal Society of Chemistry.
Citation Format(s)
Vascular lumen simulation and highly-sensitive nitric oxide detection using three-dimensional gelatin chip coupled to TiC/C nanowire arrays microelectrode. / Li, Lin-Mei; Wang, Xue-Ying; Hu, Liang-Sheng et al.
In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 12, No. 21, 07.11.2012, p. 4249-4256.
In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 12, No. 21, 07.11.2012, p. 4249-4256.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review