Using oral fluids samples for indirect influenza A virus surveillance in farmed UK pigs

Priscilla F. Gerber; Lorna Dawson; Ben Strugnell; Robert Burgess; Helen Brown; Tanja Opriessnig

doi:10.1002/vms3.51

Using oral fluids samples for indirect influenza A virus surveillance in farmed UK pigs

Priscilla F. Gerber, Lorna Dawson, Ben Strugnell, Robert Burgess, Helen Brown, Tanja Opriessnig^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

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Abstract

Influenza A virus (IAV) is economically important in pig production and has broad public health implications. In Europe, active IAV surveillance includes demonstration of antigen in nasal swabs and/or demonstration of antibodies in serum (SER) samples; however, collecting appropriate numbers of individual pig samples can be costly and labour-intensive. The objective of this study was to compare the probability of detecting IAV antibody positive populations using SER versus oral fluid (OF) samples. Paired pen samples, one OF and 5–14 SER samples, were collected cross-sectional or longitudinally. A commercial nucleoprotein (NP)-based blocking ELISA was used to test 244 OF and 1004 SER samples from 123 pens each containing 20–540 pigs located in 27 UK herds. Overall, the IAV antibody detection rate was higher in SER samples compared to OFs under the study conditions. Pig age had a significant effect on the probability of detecting positive pens. For 3–9-week-old pigs the probability of detecting IAV antibody positive samples in a pen with 95% confidence intervals was 40% (23–60) for OF and 61% (0.37–0.80) for SER (P = 0.04), for 10–14-week-old pigs it was 19% (8–40) for OF and 93% (0.71–0.99) for SER (P < 0.01), and for 18–20-week-old pigs it was 67% (41–85) for OF and 81% (0.63–0.91) for SER (P = 0.05). Collecting more than one OF sample in pens with more than 25 less than 18-week-old pigs should be further investigated in the future to elucidate the suitability of OF for IAV surveillance in herds with large pen sizes.

Original language	English
Pages (from-to)	3-12
Journal	Veterinary Medicine and Science
Volume	3
Issue number	1
DOIs	https://doi.org/10.1002/vms3.51
Publication status	Published - 1 Feb 2017
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected]

Research Keywords

diagnostics
enzyme-linked immunosorbent assay
surveillance
Swine influenza virus

Publisher's Copyright Statement

This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Using oral fluids samples for indirect influenza A virus surveillance in farmed UK pigs",

abstract = "Influenza A virus (IAV) is economically important in pig production and has broad public health implications. In Europe, active IAV surveillance includes demonstration of antigen in nasal swabs and/or demonstration of antibodies in serum (SER) samples; however, collecting appropriate numbers of individual pig samples can be costly and labour-intensive. The objective of this study was to compare the probability of detecting IAV antibody positive populations using SER versus oral fluid (OF) samples. Paired pen samples, one OF and 5–14 SER samples, were collected cross-sectional or longitudinally. A commercial nucleoprotein (NP)-based blocking ELISA was used to test 244 OF and 1004 SER samples from 123 pens each containing 20–540 pigs located in 27 UK herds. Overall, the IAV antibody detection rate was higher in SER samples compared to OFs under the study conditions. Pig age had a significant effect on the probability of detecting positive pens. For 3–9-week-old pigs the probability of detecting IAV antibody positive samples in a pen with 95% confidence intervals was 40% (23–60) for OF and 61% (0.37–0.80) for SER (P = 0.04), for 10–14-week-old pigs it was 19% (8–40) for OF and 93% (0.71–0.99) for SER (P < 0.01), and for 18–20-week-old pigs it was 67% (41–85) for OF and 81% (0.63–0.91) for SER (P = 0.05). Collecting more than one OF sample in pens with more than 25 less than 18-week-old pigs should be further investigated in the future to elucidate the suitability of OF for IAV surveillance in herds with large pen sizes.",

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AU - Gerber, Priscilla F.

AU - Dawson, Lorna

AU - Strugnell, Ben

AU - Burgess, Robert

AU - Brown, Helen

AU - Opriessnig, Tanja

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected]

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N2 - Influenza A virus (IAV) is economically important in pig production and has broad public health implications. In Europe, active IAV surveillance includes demonstration of antigen in nasal swabs and/or demonstration of antibodies in serum (SER) samples; however, collecting appropriate numbers of individual pig samples can be costly and labour-intensive. The objective of this study was to compare the probability of detecting IAV antibody positive populations using SER versus oral fluid (OF) samples. Paired pen samples, one OF and 5–14 SER samples, were collected cross-sectional or longitudinally. A commercial nucleoprotein (NP)-based blocking ELISA was used to test 244 OF and 1004 SER samples from 123 pens each containing 20–540 pigs located in 27 UK herds. Overall, the IAV antibody detection rate was higher in SER samples compared to OFs under the study conditions. Pig age had a significant effect on the probability of detecting positive pens. For 3–9-week-old pigs the probability of detecting IAV antibody positive samples in a pen with 95% confidence intervals was 40% (23–60) for OF and 61% (0.37–0.80) for SER (P = 0.04), for 10–14-week-old pigs it was 19% (8–40) for OF and 93% (0.71–0.99) for SER (P < 0.01), and for 18–20-week-old pigs it was 67% (41–85) for OF and 81% (0.63–0.91) for SER (P = 0.05). Collecting more than one OF sample in pens with more than 25 less than 18-week-old pigs should be further investigated in the future to elucidate the suitability of OF for IAV surveillance in herds with large pen sizes.

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KW - enzyme-linked immunosorbent assay

KW - surveillance

KW - Swine influenza virus

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DO - 10.1002/vms3.51

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SN - 2053-1095

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EP - 12

JO - Veterinary Medicine and Science

JF - Veterinary Medicine and Science

IS - 1

ER -

Using oral fluids samples for indirect influenza A virus surveillance in farmed UK pigs

Abstract

Bibliographical note

Research Keywords

Publisher's Copyright Statement

UN SDGs

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