USING BACTERIAL SELEX TO SELECT HIGHLY-SPECIFIC APTAMERS AND THEIR APPLICATIONS IN PAPER-BASED MICROFLUIDIC CHIPS FOR RAPID DIAGNOSIS OF MULTIPLE BACTERIA

Chih-Hung Wang; Gwo-Bin Lee

doi:10.1109/TRANSDUCERS.2017.7994149

USING BACTERIAL SELEX TO SELECT HIGHLY-SPECIFIC APTAMERS AND THEIR APPLICATIONS IN PAPER-BASED MICROFLUIDIC CHIPS FOR RAPID DIAGNOSIS OF MULTIPLE BACTERIA

Chih-Hung Wang, Gwo-Bin Lee^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

In this study, a new screening process called “bacterial SELEX” was reported to screen favorable aptamers targeting four common nosocomial and antibiotics-resistant bacteria, including Acinetobacter baumannii, vancomycin-resistant Enterococci (VRE), Escherichia coli, and multidrug-resistant Staphylococcus aureus (MRSA). The new bacterial SELEX system performed three critical selecting stages for screening of bacteria-specific aptamers. A paper-based microfluidic system was further developed by using the selected specific aptamers and a biotin-streptavidin color reaction for rapid detection of these four bacteria. The proposed paper-based microfluidic chip has several advantages, such as compactness in size, ease to handle, high specificity and capability to detect multiple pathogens simultaneously. It may be promising for point-of-care applications in the near future.

Original language	English
Title of host publication	2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)
Publisher	IEEE
Pages	718-721
ISBN (Electronic)	9781538627327
ISBN (Print)	9781538627310
DOIs	https://doi.org/10.1109/TRANSDUCERS.2017.7994149
Publication status	Published - Jun 2017
Externally published	Yes
Event	19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung Exhibition Center, Kaohsiung, Taiwan, China Duration: 18 Jun 2017 → 22 Jun 2017 http://www.transducers2017.org/

Publication series

Name	International Conference on Solid State Sensors and Actuators (TRANSDUCERS)
Publisher	IEEE
ISSN (Electronic)	2167-0021

Conference

Conference	19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
Abbreviated title	TRANSDUCERS 2017
Place	Taiwan, China
City	Kaohsiung
Period	18/06/17 → 22/06/17
Internet address	http://www.transducers2017.org/

Research Keywords

aptamer
bacterial SELEX
multidrug-resistant Staphylococcus aureus
paper-based microfluidic chip

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/TRANSDUCERS.2017.7994149

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Cite this

Wang, C.-H., & Lee, G.-B. (2017). USING BACTERIAL SELEX TO SELECT HIGHLY-SPECIFIC APTAMERS AND THEIR APPLICATIONS IN PAPER-BASED MICROFLUIDIC CHIPS FOR RAPID DIAGNOSIS OF MULTIPLE BACTERIA. In 2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS) (pp. 718-721). Article 7994149 (International Conference on Solid State Sensors and Actuators (TRANSDUCERS)). IEEE. https://doi.org/10.1109/TRANSDUCERS.2017.7994149

Wang, Chih-Hung ; Lee, Gwo-Bin. / USING BACTERIAL SELEX TO SELECT HIGHLY-SPECIFIC APTAMERS AND THEIR APPLICATIONS IN PAPER-BASED MICROFLUIDIC CHIPS FOR RAPID DIAGNOSIS OF MULTIPLE BACTERIA. 2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS) . IEEE, 2017. pp. 718-721 (International Conference on Solid State Sensors and Actuators (TRANSDUCERS)).

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abstract = "In this study, a new screening process called “bacterial SELEX” was reported to screen favorable aptamers targeting four common nosocomial and antibiotics-resistant bacteria, including Acinetobacter baumannii, vancomycin-resistant Enterococci (VRE), Escherichia coli, and multidrug-resistant Staphylococcus aureus (MRSA). The new bacterial SELEX system performed three critical selecting stages for screening of bacteria-specific aptamers. A paper-based microfluidic system was further developed by using the selected specific aptamers and a biotin-streptavidin color reaction for rapid detection of these four bacteria. The proposed paper-based microfluidic chip has several advantages, such as compactness in size, ease to handle, high specificity and capability to detect multiple pathogens simultaneously. It may be promising for point-of-care applications in the near future.",

keywords = "aptamer, bacterial SELEX, multidrug-resistant Staphylococcus aureus, paper-based microfluidic chip",

author = "Chih-Hung Wang and Gwo-Bin Lee",

year = "2017",

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doi = "10.1109/TRANSDUCERS.2017.7994149",

language = "English",

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note = "19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017, TRANSDUCERS 2017 ; Conference date: 18-06-2017 Through 22-06-2017",

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Wang, C-H & Lee, G-B 2017, USING BACTERIAL SELEX TO SELECT HIGHLY-SPECIFIC APTAMERS AND THEIR APPLICATIONS IN PAPER-BASED MICROFLUIDIC CHIPS FOR RAPID DIAGNOSIS OF MULTIPLE BACTERIA. in 2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS) ., 7994149, International Conference on Solid State Sensors and Actuators (TRANSDUCERS), IEEE, pp. 718-721, 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017, Kaohsiung, Taiwan, China, 18/06/17. https://doi.org/10.1109/TRANSDUCERS.2017.7994149

USING BACTERIAL SELEX TO SELECT HIGHLY-SPECIFIC APTAMERS AND THEIR APPLICATIONS IN PAPER-BASED MICROFLUIDIC CHIPS FOR RAPID DIAGNOSIS OF MULTIPLE BACTERIA. / Wang, Chih-Hung; Lee, Gwo-Bin.
2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS) . IEEE, 2017. p. 718-721 7994149 (International Conference on Solid State Sensors and Actuators (TRANSDUCERS)).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

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T1 - USING BACTERIAL SELEX TO SELECT HIGHLY-SPECIFIC APTAMERS AND THEIR APPLICATIONS IN PAPER-BASED MICROFLUIDIC CHIPS FOR RAPID DIAGNOSIS OF MULTIPLE BACTERIA

AU - Wang, Chih-Hung

AU - Lee, Gwo-Bin

PY - 2017/6

Y1 - 2017/6

N2 - In this study, a new screening process called “bacterial SELEX” was reported to screen favorable aptamers targeting four common nosocomial and antibiotics-resistant bacteria, including Acinetobacter baumannii, vancomycin-resistant Enterococci (VRE), Escherichia coli, and multidrug-resistant Staphylococcus aureus (MRSA). The new bacterial SELEX system performed three critical selecting stages for screening of bacteria-specific aptamers. A paper-based microfluidic system was further developed by using the selected specific aptamers and a biotin-streptavidin color reaction for rapid detection of these four bacteria. The proposed paper-based microfluidic chip has several advantages, such as compactness in size, ease to handle, high specificity and capability to detect multiple pathogens simultaneously. It may be promising for point-of-care applications in the near future.

AB - In this study, a new screening process called “bacterial SELEX” was reported to screen favorable aptamers targeting four common nosocomial and antibiotics-resistant bacteria, including Acinetobacter baumannii, vancomycin-resistant Enterococci (VRE), Escherichia coli, and multidrug-resistant Staphylococcus aureus (MRSA). The new bacterial SELEX system performed three critical selecting stages for screening of bacteria-specific aptamers. A paper-based microfluidic system was further developed by using the selected specific aptamers and a biotin-streptavidin color reaction for rapid detection of these four bacteria. The proposed paper-based microfluidic chip has several advantages, such as compactness in size, ease to handle, high specificity and capability to detect multiple pathogens simultaneously. It may be promising for point-of-care applications in the near future.

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KW - multidrug-resistant Staphylococcus aureus

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M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781538627310

T3 - International Conference on Solid State Sensors and Actuators (TRANSDUCERS)

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BT - 2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)

PB - IEEE

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Y2 - 18 June 2017 through 22 June 2017

ER -

Wang CH, Lee GB. USING BACTERIAL SELEX TO SELECT HIGHLY-SPECIFIC APTAMERS AND THEIR APPLICATIONS IN PAPER-BASED MICROFLUIDIC CHIPS FOR RAPID DIAGNOSIS OF MULTIPLE BACTERIA. In 2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS) . IEEE. 2017. p. 718-721. 7994149. (International Conference on Solid State Sensors and Actuators (TRANSDUCERS)). doi: 10.1109/TRANSDUCERS.2017.7994149

USING BACTERIAL SELEX TO SELECT HIGHLY-SPECIFIC APTAMERS AND THEIR APPLICATIONS IN PAPER-BASED MICROFLUIDIC CHIPS FOR RAPID DIAGNOSIS OF MULTIPLE BACTERIA

Abstract

Publication series

Conference

Research Keywords

UN SDGs

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