Use of sodium chloride to rapidly restore polyhydroxyalkanoates production from filamentous bulking without polyhydroxyalkanoates productivity impairment

Occurrence of filamentous bulking would lead to excess biomass washout to fail the polyhydroxyalkanoate (PHA) production process. A strategy using sodium chloride (NaCl) addition to enhance sludge settleability so that high PHA productivity could be retained was tested in two sequencing batch reactors (SBR #1 and #2) fed with different organic acid mixtures but at identical NaCl gradients (0, 5 and 10 g/L). Significant improved sludge settleability was observed at 10 g/L NaCl regardless of occurrence of filamentous bulking. The NaCl addition strategy suppressed the growth of principal filamentous bacterium, Meganema, and enriched the PHA producers (Paracoccus and Thauera) to reach enhanced PHA productivities of 0.244–0.298 g/L⁻¹ d⁻¹ for the present studied system.