Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China

L. H. Xu; P. K S Lam; J. P. Chen; J. M. Xu; B. S F Wong; Y. Y. Zhang; R. S S Wu; K. I. Harada

doi:10.1016/S0045-6535(99)00389-6

Author(s)

L. H. Xu
P. K S Lam
J. P. Chen
J. M. Xu
B. S F Wong
And 3 others

Y. Y. Zhang
R. S S Wu
K. I. Harada

Detail(s)

Original language	English
Pages (from-to)	53-58
Journal / Publication	Chemosphere
Volume	41
Issue number	1-2
Publication status	Published - Jul 2000
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1016/S0045-6535(99)00389-6 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-0342859372&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(f30339a4-9264-4dfd-b124-f48704d71a84).html

Abstract

Seasonal variations in the level of total microcystins in water samples collected from Donghu Lake and a fish pond in Wuhan, China, were studied between March 1995 and February 1996 using a protein phosphatase inhibition assay involving a radioactive ³²P-labelled substrate. The assay is highly reliable and repeatable, and is probably the most sensitive assay for microcystin detection to date. Results of the survey indicated the presence of microcystins in the water samples, and the concentration of microcystins appeared to be related to the degree of eutrophication and water temperature. There is also a correlative relationship between the quantity of microcystins and the abundance of microcystin-producing cyanobacteria (Anabaena and Oscillatoria) in the water bodies over a year cycle. In the present study, the positive detection of microcystins in water bodies having no signs of algal bloom warns of considerable potential threat of these waters to public health. (C) 2000 Elsevier Science Ltd.

Research Area(s)

SDG 14 - Life Below Water

Citation Format(s)

[ RIS ] [ BibTeX ]

Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China. / Xu, L. H.; Lam, P. K S; Chen, J. P. et al.
In: Chemosphere, Vol. 41, No. 1-2, 07.2000, p. 53-58.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Xu, LH, Lam, PKS, Chen, JP, Xu, JM, Wong, BSF, Zhang, YY, Wu, RSS & Harada, KI 2000, 'Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China', Chemosphere, vol. 41, no. 1-2, pp. 53-58. https://doi.org/10.1016/S0045-6535(99)00389-6

Xu, L. H., Lam, P. K. S., Chen, J. P., Xu, J. M., Wong, B. S. F., Zhang, Y. Y., Wu, R. S. S., & Harada, K. I. (2000). Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China. Chemosphere, 41(1-2), 53-58. https://doi.org/10.1016/S0045-6535(99)00389-6

Xu LH, Lam PKS, Chen JP, Xu JM, Wong BSF, Zhang YY et al. Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China. Chemosphere. 2000 Jul;41(1-2):53-58. doi: 10.1016/S0045-6535(99)00389-6

Xu, L. H. ; Lam, P. K S ; Chen, J. P. et al. / Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China. In: Chemosphere. 2000 ; Vol. 41, No. 1-2. pp. 53-58.