Use of IR-to-visible upconversion fluorescent nanoparticles for tracking of siRNA delivery

Shan Jiang; Yong Zhang

Use of IR-to-visible upconversion fluorescent nanoparticles for tracking of siRNA delivery

Shan Jiang
, Yong Zhang

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

1 Citation (Scopus)

Abstract

IR-to-visible upcoversion fluorescent nanoparticles (UCNs) were developed to track in vitro delivery of small interference RNA (siRNA) and meanwhile efficiently transfect siRNA to the cells. NaYF₄ upconversion nanoparticles co-doped with Yb/Tm (NaYF₄:Yb,Tm) were conjugated with GL3 siRNA through electrostatic attraction at the weight ratio of 8:3. Then, siRNA- conjugated UCNs were incubated with the cultured human breast cancer MCF-7 cells. After 24h-incubation the successful cellular uptake of siRNA was visualized through the emitted blue fluorescence of siRNA- conjugated UCNs under confocal laser scanning microscopy. After 48h-incubation the efficiency of delivered GL3 siRNA in the inhibition of luciferase gene was ~60% that was determined by luciferase assay. This article provided initial insights into the effectiveness and suitability of NaYF₄:Yb,Tm upconversion nanoparticles as an easily detectable and self-tracking delivery vector of siRNA.

Original language	English
Title of host publication	Proceedings of the 6th IASTED International Conference on Biomedical Engineering, BioMED 2008
Pages	368-371
Publication status	Published - 2008
Externally published	Yes
Event	6th IASTED International Conference on Biomedical Engineering, BioMED 2008 - Innsbruck, Austria Duration: 13 Feb 2008 → 15 Feb 2008

Publication series

Name	Proceedings of the 6th IASTED International Conference on Biomedical Engineering, BioMED 2008

Conference

Conference	6th IASTED International Conference on Biomedical Engineering, BioMED 2008
Place	Austria
City	Innsbruck
Period	13/02/08 → 15/02/08

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

Biomaterials
Imaging
Sirna delivery
Upconversion nanoparticles

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Cite this

@inproceedings{388093ba52344a8bbcece166501777c8,

title = "Use of IR-to-visible upconversion fluorescent nanoparticles for tracking of siRNA delivery",

abstract = "IR-to-visible upcoversion fluorescent nanoparticles (UCNs) were developed to track in vitro delivery of small interference RNA (siRNA) and meanwhile efficiently transfect siRNA to the cells. NaYF4 upconversion nanoparticles co-doped with Yb/Tm (NaYF4:Yb,Tm) were conjugated with GL3 siRNA through electrostatic attraction at the weight ratio of 8:3. Then, siRNA- conjugated UCNs were incubated with the cultured human breast cancer MCF-7 cells. After 24h-incubation the successful cellular uptake of siRNA was visualized through the emitted blue fluorescence of siRNA- conjugated UCNs under confocal laser scanning microscopy. After 48h-incubation the efficiency of delivered GL3 siRNA in the inhibition of luciferase gene was \textasciitilde{}60\% that was determined by luciferase assay. This article provided initial insights into the effectiveness and suitability of NaYF4:Yb,Tm upconversion nanoparticles as an easily detectable and self-tracking delivery vector of siRNA.",

keywords = "Biomaterials, Imaging, Sirna delivery, Upconversion nanoparticles",

author = "Shan Jiang and Yong Zhang",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected]. ; 6th IASTED International Conference on Biomedical Engineering, BioMED 2008 ; Conference date: 13-02-2008 Through 15-02-2008",

year = "2008",

language = "English",

isbn = "9780889867215",

series = "Proceedings of the 6th IASTED International Conference on Biomedical Engineering, BioMED 2008",

pages = "368--371",

booktitle = "Proceedings of the 6th IASTED International Conference on Biomedical Engineering, BioMED 2008",

}

Jiang, S & Zhang, Y 2008, Use of IR-to-visible upconversion fluorescent nanoparticles for tracking of siRNA delivery. in Proceedings of the 6th IASTED International Conference on Biomedical Engineering, BioMED 2008. Proceedings of the 6th IASTED International Conference on Biomedical Engineering, BioMED 2008, pp. 368-371, 6th IASTED International Conference on Biomedical Engineering, BioMED 2008, Innsbruck, Austria, 13/02/08.

Use of IR-to-visible upconversion fluorescent nanoparticles for tracking of siRNA delivery. / Jiang, Shan; Zhang, Yong.
Proceedings of the 6th IASTED International Conference on Biomedical Engineering, BioMED 2008. 2008. p. 368-371 (Proceedings of the 6th IASTED International Conference on Biomedical Engineering, BioMED 2008).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Use of IR-to-visible upconversion fluorescent nanoparticles for tracking of siRNA delivery

AU - Jiang, Shan

AU - Zhang, Yong

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2008

Y1 - 2008

N2 - IR-to-visible upcoversion fluorescent nanoparticles (UCNs) were developed to track in vitro delivery of small interference RNA (siRNA) and meanwhile efficiently transfect siRNA to the cells. NaYF4 upconversion nanoparticles co-doped with Yb/Tm (NaYF4:Yb,Tm) were conjugated with GL3 siRNA through electrostatic attraction at the weight ratio of 8:3. Then, siRNA- conjugated UCNs were incubated with the cultured human breast cancer MCF-7 cells. After 24h-incubation the successful cellular uptake of siRNA was visualized through the emitted blue fluorescence of siRNA- conjugated UCNs under confocal laser scanning microscopy. After 48h-incubation the efficiency of delivered GL3 siRNA in the inhibition of luciferase gene was ~60% that was determined by luciferase assay. This article provided initial insights into the effectiveness and suitability of NaYF4:Yb,Tm upconversion nanoparticles as an easily detectable and self-tracking delivery vector of siRNA.

AB - IR-to-visible upcoversion fluorescent nanoparticles (UCNs) were developed to track in vitro delivery of small interference RNA (siRNA) and meanwhile efficiently transfect siRNA to the cells. NaYF4 upconversion nanoparticles co-doped with Yb/Tm (NaYF4:Yb,Tm) were conjugated with GL3 siRNA through electrostatic attraction at the weight ratio of 8:3. Then, siRNA- conjugated UCNs were incubated with the cultured human breast cancer MCF-7 cells. After 24h-incubation the successful cellular uptake of siRNA was visualized through the emitted blue fluorescence of siRNA- conjugated UCNs under confocal laser scanning microscopy. After 48h-incubation the efficiency of delivered GL3 siRNA in the inhibition of luciferase gene was ~60% that was determined by luciferase assay. This article provided initial insights into the effectiveness and suitability of NaYF4:Yb,Tm upconversion nanoparticles as an easily detectable and self-tracking delivery vector of siRNA.

KW - Biomaterials

KW - Imaging

KW - Sirna delivery

KW - Upconversion nanoparticles

UR - https://www.scopus.com/pages/publications/62949087451

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-62949087451&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9780889867215

T3 - Proceedings of the 6th IASTED International Conference on Biomedical Engineering, BioMED 2008

SP - 368

EP - 371

BT - Proceedings of the 6th IASTED International Conference on Biomedical Engineering, BioMED 2008

T2 - 6th IASTED International Conference on Biomedical Engineering, BioMED 2008

Y2 - 13 February 2008 through 15 February 2008

ER -

Use of IR-to-visible upconversion fluorescent nanoparticles for tracking of siRNA delivery

Abstract

Publication series

Conference

Bibliographical note

UN SDGs

Research Keywords

Other Access Options

Permanent Link

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