@inbook{795e1331a3dc4e03a6322496776194cc,
title = "Use of interim scaffolding and neotissue development to produce a scaffold-free living hyaline cartilage graft",
abstract = "The fabrication of three-dimensional (3D) constructs relies heavily on the use of biomaterial-based scaffolds. These are required as mechanical supports as well as to translate two-dimensional cultures to 3D cultures for clinical applications. Regardless of the choice of scaffold, timely degradation of scaffolds is difficult to achieve and undegraded scaffold material can lead to interference in further tissue development or morphogenesis. In cartilage tissue engineering, hydrogel is the highly preferred scaffold material as it shares many similar characteristics with native cartilaginous matrix. Hence, we employed gelatin microspheres as porogens to create a microcavitary alginate hydrogel as an interim scaffold to facilitate initial chondrocyte 3D culture and to establish a final scaffold-free living hyaline cartilaginous graft (LhCG) for cartilage tissue engineering",
keywords = "Biomaterials, Cartilage, Hydrogel, Scaffold-free, Tissue engineering",
author = "Lau, \{Ting Ting\} and Wenyan Leong and Yvonne Peck and Kai Su and Dong-An Wang",
note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to
[email protected].",
year = "2015",
doi = "10.1007/978-1-4939-2938-2\_10",
language = "English",
volume = "1340",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "153--160",
booktitle = "Methods in Molecular Biology",
}