Use of a whole chromosomal probe for identification of Treponema hyodysenteriae

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Original languageEnglish
Pages (from-to)286-289
Journal / PublicationResearch in Veterinary Science
Volume50
Issue number3
Publication statusPublished - May 1991
Externally publishedYes

Abstract

A whole chromosomal DNA probe labelled with photobiotin was used in a dot blot hybridisation to identify DNA from isolates of Treponema hyodysenteriae, the aetiological agent of swine dysentery. The probe was evaluated using DNA from 13 isolates of T hyodysenteriae and 13 isolates of non-T hyodysenteriae spirochaetes recovered from pigs. The initial test had both a sensitivity and specificity of 92·3 per cent, although when it was repeated the specificity fell to 84·6 per cent. The test was helpful in distinguishing between T hyodysenteriae and other morphologically similar treponemes that are part of the normal flora in the large intestine of pigs. The probe could also be used to detect as little as 10 ng of purified DNA from T hyodysenteriae, or DNA from 2 × 106 bacterial cells lysed directly onto nitrocellulose.