Upregulation of intestinal NHE3 following saline ingestion

Venkanna Pasham; Anand Rotte; Shuchen Gu; Wenting Yang; Madhuri Bhandaru; Rexhep Rexhepaj; Ganesh Pathare; Florian Lang

doi:10.1159/000343401

Upregulation of intestinal NHE3 following saline ingestion

Venkanna Pasham
, Anand Rotte
, Shuchen Gu
, Wenting Yang
, Madhuri Bhandaru
, Rexhep Rexhepaj
, Ganesh Pathare
, Florian Lang

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

15 Citations (Scopus)

Abstract

Background: Little is known about the effect of salt content of ingested fluid on intestinal transport processes. Osmosensitive genes include the serum-and glucocorticoid-inducible kinase SGK1, which is up-regulated by hyperosmolarity and cell shrinkage. SGK1 is in turn a powerful stimulator of the intestinal Na⁺/H⁺ exchanger NHE3. The present study was thus performed to elucidate, whether the NaCl content of beverages influences NHE3 activity. Methods: Mice were offered access to either plain water or isotonic saline ad libitum. NHE3 transcript levels and protein abundance in intestinal tissue were determined by confocal immunofluorescent microscopy, RT-PCR and western blotting, cytosolic pH (pH_i) in intestinal cells from 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) fluorescence and Na⁺/H⁺ exchanger activity from the Na⁺ dependent realkalinization following an ammonium pulse. Results: Saline drinking significantly enhanced fluid intake and increased NHE3 transcript levels, NHE3 protein and Na⁺/H⁺ exchanger activity. Conclusions: Salt content of ingested fluid has a profound effect on intestinal Na⁺/H⁺ exchanger expression and activity. © 2013 S. Karger AG, Basel.

Original language	English
Pages (from-to)	48-57
Journal	Kidney and Blood Pressure Research
Volume	37
Issue number	1
DOIs	https://doi.org/10.1159/000343401
Publication status	Published - Apr 2013
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

The authors gratefully acknowledge the meticulous preparation of the manuscript by Lejla Subasic. The study has been supported by DFG and Open Access Publishing Fund of Tuebingen University.

Research Keywords

Fluid intake
Na+/H+ exchanger
Osmolarity
Salt
Water

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This full text is made available under CC-BY-NC 3.0. https://creativecommons.org/licenses/by-nc/3.0/

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title = "Upregulation of intestinal NHE3 following saline ingestion",

abstract = "Background: Little is known about the effect of salt content of ingested fluid on intestinal transport processes. Osmosensitive genes include the serum-and glucocorticoid-inducible kinase SGK1, which is up-regulated by hyperosmolarity and cell shrinkage. SGK1 is in turn a powerful stimulator of the intestinal Na+/H+ exchanger NHE3. The present study was thus performed to elucidate, whether the NaCl content of beverages influences NHE3 activity. Methods: Mice were offered access to either plain water or isotonic saline ad libitum. NHE3 transcript levels and protein abundance in intestinal tissue were determined by confocal immunofluorescent microscopy, RT-PCR and western blotting, cytosolic pH (pHi) in intestinal cells from 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) fluorescence and Na+/H+ exchanger activity from the Na+ dependent realkalinization following an ammonium pulse. Results: Saline drinking significantly enhanced fluid intake and increased NHE3 transcript levels, NHE3 protein and Na+/H+ exchanger activity. Conclusions: Salt content of ingested fluid has a profound effect on intestinal Na+/H+ exchanger expression and activity. {\textcopyright} 2013 S. Karger AG, Basel.",

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AU - Rotte, Anand

AU - Gu, Shuchen

AU - Yang, Wenting

AU - Bhandaru, Madhuri

AU - Rexhepaj, Rexhep

AU - Pathare, Ganesh

AU - Lang, Florian

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N2 - Background: Little is known about the effect of salt content of ingested fluid on intestinal transport processes. Osmosensitive genes include the serum-and glucocorticoid-inducible kinase SGK1, which is up-regulated by hyperosmolarity and cell shrinkage. SGK1 is in turn a powerful stimulator of the intestinal Na+/H+ exchanger NHE3. The present study was thus performed to elucidate, whether the NaCl content of beverages influences NHE3 activity. Methods: Mice were offered access to either plain water or isotonic saline ad libitum. NHE3 transcript levels and protein abundance in intestinal tissue were determined by confocal immunofluorescent microscopy, RT-PCR and western blotting, cytosolic pH (pHi) in intestinal cells from 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) fluorescence and Na+/H+ exchanger activity from the Na+ dependent realkalinization following an ammonium pulse. Results: Saline drinking significantly enhanced fluid intake and increased NHE3 transcript levels, NHE3 protein and Na+/H+ exchanger activity. Conclusions: Salt content of ingested fluid has a profound effect on intestinal Na+/H+ exchanger expression and activity. © 2013 S. Karger AG, Basel.

AB - Background: Little is known about the effect of salt content of ingested fluid on intestinal transport processes. Osmosensitive genes include the serum-and glucocorticoid-inducible kinase SGK1, which is up-regulated by hyperosmolarity and cell shrinkage. SGK1 is in turn a powerful stimulator of the intestinal Na+/H+ exchanger NHE3. The present study was thus performed to elucidate, whether the NaCl content of beverages influences NHE3 activity. Methods: Mice were offered access to either plain water or isotonic saline ad libitum. NHE3 transcript levels and protein abundance in intestinal tissue were determined by confocal immunofluorescent microscopy, RT-PCR and western blotting, cytosolic pH (pHi) in intestinal cells from 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) fluorescence and Na+/H+ exchanger activity from the Na+ dependent realkalinization following an ammonium pulse. Results: Saline drinking significantly enhanced fluid intake and increased NHE3 transcript levels, NHE3 protein and Na+/H+ exchanger activity. Conclusions: Salt content of ingested fluid has a profound effect on intestinal Na+/H+ exchanger expression and activity. © 2013 S. Karger AG, Basel.

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Upregulation of intestinal NHE3 following saline ingestion

Abstract

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Research Keywords

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