Ultraviolet damages sperm mitochondrial function and membrane integrity in the sea urchin Anthocidaris crassispina

X. Y. Lu; R. S.S. Wu

doi:10.1016/j.ecoenv.2004.12.012

Ultraviolet damages sperm mitochondrial function and membrane integrity in the sea urchin Anthocidaris crassispina

X. Y. Lu
, R. S.S. Wu

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

36 Citations (Scopus)

Abstract

Effects of ultraviolet A (UVA) and ultraviolet B (UVB) on mitochondrial function and membrane integrity of sea urchin sperm were investigated using flow cytometry and fluorescent probes. Both UVA and UVB impaired sperm mitochondrial function in a dose-dependent manner. Covariance analysis further showed that the slopes of change in mitochondrial function in relation to UVA and UVB were significantly different, suggesting that the modes of action were different. UVA did not affect membrane integrity, while membrane integrity showed a linear reduction with increasing UVB doses. Sperm mitochondria function showed significant positive correlations with sperm motility and subsequent fertilization success. Overall, our results showed that both UVA and UVB could decrease sperm motility and fertilization success through impairment of mitochondrial function, whereas UVB alone could cause additional damage through impairing the functional integrity of sperm membrane. Mitochondrial function of sperm may also offer a reliable ecotoxicological biomarker for predicting fertilization success in urchins. © 2005 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	53-59
Journal	Ecotoxicology and Environmental Safety
Volume	61
Issue number	1
DOIs	https://doi.org/10.1016/j.ecoenv.2004.12.012
Publication status	Published - 2005

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Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

This research was fully supported by a grant from the Research Grants Council of the Hong Kong Special Administrative Region, China (9040655 CityU 1091/01 M).

Research Keywords

Flow cytometry
Membrane integrity
Mitochondrial function
Sea urchin
UVA
UVB

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title = "Ultraviolet damages sperm mitochondrial function and membrane integrity in the sea urchin Anthocidaris crassispina",

abstract = "Effects of ultraviolet A (UVA) and ultraviolet B (UVB) on mitochondrial function and membrane integrity of sea urchin sperm were investigated using flow cytometry and fluorescent probes. Both UVA and UVB impaired sperm mitochondrial function in a dose-dependent manner. Covariance analysis further showed that the slopes of change in mitochondrial function in relation to UVA and UVB were significantly different, suggesting that the modes of action were different. UVA did not affect membrane integrity, while membrane integrity showed a linear reduction with increasing UVB doses. Sperm mitochondria function showed significant positive correlations with sperm motility and subsequent fertilization success. Overall, our results showed that both UVA and UVB could decrease sperm motility and fertilization success through impairment of mitochondrial function, whereas UVB alone could cause additional damage through impairing the functional integrity of sperm membrane. Mitochondrial function of sperm may also offer a reliable ecotoxicological biomarker for predicting fertilization success in urchins. {\textcopyright} 2005 Elsevier Inc. All rights reserved.",

keywords = "Flow cytometry, Membrane integrity, Mitochondrial function, Sea urchin, UVA, UVB",

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AU - Lu, X. Y.

AU - Wu, R. S.S.

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2005

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N2 - Effects of ultraviolet A (UVA) and ultraviolet B (UVB) on mitochondrial function and membrane integrity of sea urchin sperm were investigated using flow cytometry and fluorescent probes. Both UVA and UVB impaired sperm mitochondrial function in a dose-dependent manner. Covariance analysis further showed that the slopes of change in mitochondrial function in relation to UVA and UVB were significantly different, suggesting that the modes of action were different. UVA did not affect membrane integrity, while membrane integrity showed a linear reduction with increasing UVB doses. Sperm mitochondria function showed significant positive correlations with sperm motility and subsequent fertilization success. Overall, our results showed that both UVA and UVB could decrease sperm motility and fertilization success through impairment of mitochondrial function, whereas UVB alone could cause additional damage through impairing the functional integrity of sperm membrane. Mitochondrial function of sperm may also offer a reliable ecotoxicological biomarker for predicting fertilization success in urchins. © 2005 Elsevier Inc. All rights reserved.

AB - Effects of ultraviolet A (UVA) and ultraviolet B (UVB) on mitochondrial function and membrane integrity of sea urchin sperm were investigated using flow cytometry and fluorescent probes. Both UVA and UVB impaired sperm mitochondrial function in a dose-dependent manner. Covariance analysis further showed that the slopes of change in mitochondrial function in relation to UVA and UVB were significantly different, suggesting that the modes of action were different. UVA did not affect membrane integrity, while membrane integrity showed a linear reduction with increasing UVB doses. Sperm mitochondria function showed significant positive correlations with sperm motility and subsequent fertilization success. Overall, our results showed that both UVA and UVB could decrease sperm motility and fertilization success through impairment of mitochondrial function, whereas UVB alone could cause additional damage through impairing the functional integrity of sperm membrane. Mitochondrial function of sperm may also offer a reliable ecotoxicological biomarker for predicting fertilization success in urchins. © 2005 Elsevier Inc. All rights reserved.

KW - Flow cytometry

KW - Membrane integrity

KW - Mitochondrial function

KW - Sea urchin

KW - UVA

KW - UVB

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DO - 10.1016/j.ecoenv.2004.12.012

M3 - RGC 21 - Publication in refereed journal

C2 - 15814310

SN - 0147-6513

VL - 61

SP - 53

EP - 59

JO - Ecotoxicology and Environmental Safety

JF - Ecotoxicology and Environmental Safety

IS - 1

ER -

Ultraviolet damages sperm mitochondrial function and membrane integrity in the sea urchin Anthocidaris crassispina

Abstract

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Research Keywords

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