Ultra-High Throughput Enrichment of Viable Circulating Tumor Cells

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review

2 Scopus Citations
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Author(s)

  • M.E. Warkiani
  • Guofeng Guan
  • Daniel Shao-Weng Tan
  • Alvin S.T. Lim
  • Wan-Teck Lim
  • Yoon Sim Yap
  • Soo Chin Lee
  • Ross A. Soo
  • Jongyoon Han
  • Chwee Teck Lim

Detail(s)

Original languageEnglish
Title of host publicationThe 15th International Conference on Biomedical Engineering
Subtitle of host publicationICBME 2013
EditorsJames Goh
PublisherSpringer
Pages1-4
ISBN (Electronic)9783319029139
ISBN (Print)9783319029122
Publication statusPublished - Dec 2013
Externally publishedYes

Publication series

NameIFMBE Proceedings
PublisherSpringer
Volume43
ISSN (Print)1680-0737
ISSN (Electronic)1433-9277

Conference

Title15th International Conference on Biomedical Engineering (ICBME 2013)
PlaceSingapore
Period4 - 7 December 2013

Abstract

Detection, enumeration and characterization of rare circulating tumor cells (CTCs) from the peripheral blood of cancer patients potentially provide critical insights into tumor biology and is promising for cancer diagnosis and prognosis. Here, we present a novel multiplexed spiral microfluidic device for ultra-high throughput, label-free enrichment of CTCs from clinically relevant blood volumes. The fast processing time of the technique (7.5 mL blood in < 5 min) and high sensitivity of the device lends itself to a broad range of potential genomic and transcriptomic applications. The method can specifically separate and preserve all fractions of blood (i.e., plasma, CTCs and PBMC) for diverse downstream analysis. CTCs were detected from 100% (10/10) of blood samples collected from patients with advanced stage metastatic breast (12- 56 CTC/ml) or lung cancer (30-153 CTC/ml). Cancer cells were characterized with immunostaining and fluorescence in situ hybridization (FISH) (HER2/neu). Retrieved cells were unlabelled and hence more viable for propagation and other informative analysis such as the next generation sequencing (NGS) to guide treatment and individualized patient care.

Research Area(s)

  • Cancer detection, Cell separation, Circulating tumor cells (CTCs), Inertial microfluidics, Point-of-care diagnostics

Citation Format(s)

Ultra-High Throughput Enrichment of Viable Circulating Tumor Cells. / Khoo, B.L.; Warkiani, M.E.; Guan, Guofeng et al.

The 15th International Conference on Biomedical Engineering: ICBME 2013. ed. / James Goh. Springer, 2013. p. 1-4 (IFMBE Proceedings; Vol. 43).

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review