Ultrabright and ultrastable near-infrared dye nanoparticles for in vitro and in vivo bioimaging

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Yinlong Yang
  • Feifei An
  • Zhuang Liu
  • Xiujuan Zhang
  • Mengjiao Zhou
  • Wei Li
  • Xiaojun Hao
  • Xiaohong Zhang

Detail(s)

Original languageEnglish
Pages (from-to)7803-7809
Journal / PublicationBiomaterials
Volume33
Issue number31
Publication statusPublished - Nov 2012

Abstract

We report a new strategy of using carrier-free pure near-infrared (NIR) dye nanoparticles (NPs) to achieve highly luminescent NIR fluorescent probes for in vitro and in vivo imaging. Bis(4-(N-(2-naphthyl)phenylamino) phenyl)-fumaronitrile (NPAPF) NPs are shown to exhibit favorable biocompatibility, wide-range pH stability (pH 4-10) and much more superior photostability than conventional dyes. Importantly, the combined merits of high dye loading content and aggregation-induced emission enhancement properties, endow the NIR probes with high brightness and a high quantum yield up to 14.9%. The NPAPF NPs can be readily conjugated with folic acid for targeted in vitro cell imaging. Applications of the NPs probes in high efficiency in vivo and ex vivo imaging were successfully demonstrated. Intense fluorescent signals of NPAPF NPs can be distinctly, selectively and spatially resolved in tumor sites with ultrahigh sensitivity, even with 5 ms exposure time, due to the preferentially accumulation of NPs in tumor sites through passive enhanced permeability and retention effect. The totality of results clearly demonstrate the exciting potential of the functionalized NPAPF NPs as a NIR fluorescent probe for in vitro and in vivo imaging and diagnostics. andcopy; 2012 Elsevier Ltd.

Research Area(s)

  • Fluorescence nanoparticles, In vitro imaging, In vivo imaging, Near-infrared probes

Citation Format(s)

Ultrabright and ultrastable near-infrared dye nanoparticles for in vitro and in vivo bioimaging. / Yang, Yinlong; An, Feifei; Liu, Zhuang; Zhang, Xiujuan; Zhou, Mengjiao; Li, Wei; Hao, Xiaojun; Lee, Chun-Sing; Zhang, Xiaohong.

In: Biomaterials, Vol. 33, No. 31, 11.2012, p. 7803-7809.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review