Two-dimensional microfluidic bioarray for nucleic acid analysis

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 12 - Chapter in an edited book (Author)peer-review

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Original languageEnglish
Title of host publicationIntegrated Microsystems: Electronics, Photonics, and Biotechnology
PublisherCRC Press
ISBN (print)9781439836217, 9781439836200
Publication statusPublished - 1 Jan 2017
Externally publishedYes


Nucleic acid hybridization techniques feature the use of a probe nucleic acid molecule to detect a target nucleic acid molecule. Here, probe molecules are usually short single-stranded nucleic acids (DNA or RNA) or oligonucleotides with known sequences; whereas target molecules are prepared from polymerase chain reaction (PCR) amplification of genomic extracts. Probe-target hybridization leads to the formation of a double-stranded molecule, called duplex. The concept of DNA microarray was evolved from Southern blotting technology based on solid-phase hybridization in the early 1990s [1]. This method relies on the immobilization of the probe molecules onto the solid surface to recognize their complementary DNA target sequence by hybridization. Up to millions of features have been integrated into a standard glass slide or silicon chip by microprinting or in situ synthesis of oligonucleotides [2,3]. The relative abundance of nucleic acid sequences in the target can be measured from chip-hybridization results optically, electrochemically, or radioactively, with proper detection labels [4]. DNA microarrays have dramatically accelerated many types of investigations including gene expression profiling, comparative genomic hybridization, protein-DNA interaction study (chromatin immunoprecipitation), single-nucleotide polymorphism (SNP) detection, and nucleic acid diagnostic applications. The advances in DNA microarray technology during the last couple of years have been summarized in many books and reviews [4-8].

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Citation Format(s)

Two-dimensional microfluidic bioarray for nucleic acid analysis. / Wang, Lin; Li, Paul C.H.
Integrated Microsystems: Electronics, Photonics, and Biotechnology. CRC Press, 2017. p. 563-580.

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 12 - Chapter in an edited book (Author)peer-review