Two Rab2 interactors regulate dense-core vesicle maturation

Michael Ailion; Mandy Hannemann; Susan Dalton; Andrea Pappas; Shigeki Watanabe; Jan Hegermann; Qiang Liu; Hsiao-Fen Han; Mingyu Gu; Morgan Q. Goulding; Nikhil Sasidharan; Kim Schuske; Patrick Hullett; Stefan Eimer; Erik M. Jorgensen

doi:10.1016/j.neuron.2014.02.017

Two Rab2 interactors regulate dense-core vesicle maturation

Michael Ailion
, Mandy Hannemann
, Susan Dalton
, Andrea Pappas
, Shigeki Watanabe
, Jan Hegermann
, Qiang Liu
, Hsiao-Fen Han
, Mingyu Gu
, Morgan Q. Goulding
, Nikhil Sasidharan
, Kim Schuske
, Patrick Hullett
, Stefan Eimer
, Erik M. Jorgensen

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

62 Citations (Scopus)

Abstract

Peptide neuromodulators are released from a unique organelle: the dense-core vesicle. Dense-core vesicles are generated at the trans-Golgi and then sort cargo during maturation before being secreted. To identify proteins that act in this pathway, we performed a genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We identified two conserved Rab2-binding proteins: RUND-1, a RUN domain protein, and CCCP-1, a coiled-coil protein. RUND-1 and CCCP-1 colocalize with RAB-2 at the Golgi, and rab-2, rund-1, and cccp-1 mutants have similar defects in sorting soluble and transmembrane dense-core vesicle cargos. RUND-1 also interacts with the Rab2 GAP protein TBC-8 and the BAR domain protein RIC-19, a RAB-2 effector. In summary, a pathway of conserved proteins controls the maturation of dense-core vesicles at the trans-Golgi network. © 2014 Elsevier Inc.

Original language	English
Pages (from-to)	167-180
Journal	Neuron
Volume	82
Issue number	1
DOIs	https://doi.org/10.1016/j.neuron.2014.02.017
Publication status	Published - 2 Apr 2014
Externally published	Yes

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Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

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title = "Two Rab2 interactors regulate dense-core vesicle maturation",

abstract = "Peptide neuromodulators are released from a unique organelle: the dense-core vesicle. Dense-core vesicles are generated at the trans-Golgi and then sort cargo during maturation before being secreted. To identify proteins that act in this pathway, we performed a genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We identified two conserved Rab2-binding proteins: RUND-1, a RUN domain protein, and CCCP-1, a coiled-coil protein. RUND-1 and CCCP-1 colocalize with RAB-2 at the Golgi, and rab-2, rund-1, and cccp-1 mutants have similar defects in sorting soluble and transmembrane dense-core vesicle cargos. RUND-1 also interacts with the Rab2 GAP protein TBC-8 and the BAR domain protein RIC-19, a RAB-2 effector. In summary, a pathway of conserved proteins controls the maturation of dense-core vesicles at the trans-Golgi network. {\textcopyright} 2014 Elsevier Inc.",

author = "Michael Ailion and Mandy Hannemann and Susan Dalton and Andrea Pappas and Shigeki Watanabe and Jan Hegermann and Qiang Liu and Hsiao-Fen Han and Mingyu Gu and Goulding, \{Morgan Q.\} and Nikhil Sasidharan and Kim Schuske and Patrick Hullett and Stefan Eimer and Jorgensen, \{Erik M.\}",

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AU - Ailion, Michael

AU - Hannemann, Mandy

AU - Dalton, Susan

AU - Pappas, Andrea

AU - Watanabe, Shigeki

AU - Hegermann, Jan

AU - Liu, Qiang

AU - Han, Hsiao-Fen

AU - Gu, Mingyu

AU - Goulding, Morgan Q.

AU - Sasidharan, Nikhil

AU - Schuske, Kim

AU - Hullett, Patrick

AU - Eimer, Stefan

AU - Jorgensen, Erik M.

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PY - 2014/4/2

Y1 - 2014/4/2

N2 - Peptide neuromodulators are released from a unique organelle: the dense-core vesicle. Dense-core vesicles are generated at the trans-Golgi and then sort cargo during maturation before being secreted. To identify proteins that act in this pathway, we performed a genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We identified two conserved Rab2-binding proteins: RUND-1, a RUN domain protein, and CCCP-1, a coiled-coil protein. RUND-1 and CCCP-1 colocalize with RAB-2 at the Golgi, and rab-2, rund-1, and cccp-1 mutants have similar defects in sorting soluble and transmembrane dense-core vesicle cargos. RUND-1 also interacts with the Rab2 GAP protein TBC-8 and the BAR domain protein RIC-19, a RAB-2 effector. In summary, a pathway of conserved proteins controls the maturation of dense-core vesicles at the trans-Golgi network. © 2014 Elsevier Inc.

AB - Peptide neuromodulators are released from a unique organelle: the dense-core vesicle. Dense-core vesicles are generated at the trans-Golgi and then sort cargo during maturation before being secreted. To identify proteins that act in this pathway, we performed a genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We identified two conserved Rab2-binding proteins: RUND-1, a RUN domain protein, and CCCP-1, a coiled-coil protein. RUND-1 and CCCP-1 colocalize with RAB-2 at the Golgi, and rab-2, rund-1, and cccp-1 mutants have similar defects in sorting soluble and transmembrane dense-core vesicle cargos. RUND-1 also interacts with the Rab2 GAP protein TBC-8 and the BAR domain protein RIC-19, a RAB-2 effector. In summary, a pathway of conserved proteins controls the maturation of dense-core vesicles at the trans-Golgi network. © 2014 Elsevier Inc.

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VL - 82

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JO - Neuron

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Two Rab2 interactors regulate dense-core vesicle maturation

Abstract

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