Abstract
The hairpin element (HpE) near the start codon in the 5′ untranslated region (UTR) was developed to tune the mRNA translation in mammalian cells. The parameters of HpEs including thermodynamic stability, the content of guanine and cytosine, and distance between HpEs and the 5′ cap were investigated. These parameters influenced the mRNA expression level. In addition, the start codon and the upstream open reading frame sequestered within the structures of HpEs also reduced the translation initiation. In summary, this study shows that the simple engineering HpE structure can be efficiently adopted for gene expression regulation. The predictable controllability of this simple cloning strategy can potentially achieve precise gene expression regulation in different mammalian cell types. © 2023 Chinese Physical Society.
| Translated title of the contribution | 哺乳动物细胞中 mRNA 起始密码子附近的发夹组件对基因表达的调控 |
|---|---|
| Original language | English |
| Pages (from-to) | 755-763 |
| Journal | Chinese Journal of Chemical Physics |
| Volume | 36 |
| Issue number | 6 |
| Online published | 1 Dec 2023 |
| DOIs | |
| Publication status | Published - Dec 2023 |
Research Keywords
- Gene expression regulation
- Hairpin element
- mRNA
- Start codon
Publisher's Copyright Statement
- COPYRIGHT TERMS OF DEPOSITED FINAL PUBLISHED VERSION FILE: This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared Xue Yin, Dong-bao Yao, Hao-jun Liang; Tuning gene expression by hairpin elements near the start codon of mRNA in mammalian cells. Chin. J. Chem. Phys. 1 December 2023; 36 (6): 755–763 and may be found at https://doi.org/10.1063/1674-0068/cjcp2207107.