TRPV4, TRPC1, and TRPP2 assemble to form a flow-sensitive heteromeric channel

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Juan Du
  • Xin Ma
  • Bing Shen
  • Lutz Birnbaumer
  • Xiaoqiang Yao

Detail(s)

Original languageEnglish
Pages (from-to)4677-4685
Journal / PublicationFASEB Journal
Volume28
Issue number11
Publication statusPublished - 1 Nov 2014
Externally publishedYes

Abstract

Transient receptor potential (TRP) channels, a superfamily of ion channels, can be divided into 7 subfamilies, including TRPV, TRPC, TRPP, and 4 others. Functional TRP channels are tetrameric complexes consisting of 4 pore-forming subunits. The purpose of this study was to explore the heteromerization of TRP subunits crossing different TRP subfamilies. Two-step coimmunoprecipitation (co-IP) and fluorescence resonance energy transfer (FRET) were used to determine the interaction of the different TRP subunits. Patch-clamp and cytosolic Ca2+ measurements were used to determine the functional role of the ion channels in flow conditions. The analysis demonstrated the formation of a heteromeric TRPV4-C1-P2 complex in primary cultured rat mesenteric artery endothelial cells (MAECs) and HEK293 cells that were cotransfected with TRPV4, TRPC1, and TRPP2. In functional experiments, pore-dead mutants for each of these 3 TRP isoforms nearly abolished the flow-induced cation currents and Ca2+ increase, suggesting that all 3 TRPs contribute to the ion permeation pore of the channels. We identified the first heteromeric TRP channels composed of subunits from 3 different TRP subfamilies. Functionally, this heteromeric TRPV4- C1-P2 channel mediates the flow-induced Ca2+ increase in native vascular endothelial cells.-Du, J., Ma, X., Shen, B., Huang, Y., Birnbaumer, L., Yao, X. TRPV4, TRPC1, and TRPP2 assemble to form a flowsensitive heteromeric channel.

Research Area(s)

  • Ca2+, Endothelial cells, Mesenteric artery, Shear stress

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to lbscholars@cityu.edu.hk.

Citation Format(s)

TRPV4, TRPC1, and TRPP2 assemble to form a flow-sensitive heteromeric channel. / Du, Juan; Ma, Xin; Shen, Bing et al.
In: FASEB Journal, Vol. 28, No. 11, 01.11.2014, p. 4677-4685.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review