Transposon-associated lincosamide resistance lnu (C) gene identified in Brachyspira hyodysenteriae ST83

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

11 Scopus Citations
View graph of relations

Author(s)

  • Silvio De Luca
  • Pamela Nicholson
  • Chiara F. Magistrali
  • Ana B. García-Martín
  • Lorenz Rychener
  • Joachim Frey
  • Vincent Perreten

Detail(s)

Original languageEnglish
Pages (from-to)51-55
Journal / PublicationVeterinary Microbiology
Volume214
Online published7 Dec 2017
Publication statusPublished - Feb 2018
Externally publishedYes

Abstract

Treatment of Swine Dysentery (SD) caused by Brachyspira hyodysenteriae (B. hyodysenteriae) is carried out using antimicrobials such as macrolides, lincosamides and pleuromutilins leading to the selection of resistant strains. Whole genome sequencing of a multidrug-resistant B. hyodysenteriae strain called BH718 belonging to sequence type (ST) 83 revealed the presence of the lincosamide resistance gene lnu (C) on the small 1724-bp transposon MTnSag1. The strain also contains an A to T substitution at position 2058 (A2058T) in the 23S rRNA gene which is known to be associated with macrolide and lincosamide resistance in B. hyodysenteriae. Testing of additional strains showed that those containing lnu(C) exhibited a higher minimal inhibitory concentration (MIC) of lincomycin (MIC ≥ 64 mg/L) compared to strains lacking lnu (C), even if they also harbor the A2058T mutation. Resistance to pleuromutilins could not be explained by the presence of already reported mutations in the 23S rRNA gene and in the ribosomal protein L3. This study shows that B. hyodysenteriae has the ability to acquire mobile genetic elements conferring resistance to antibiotics.

Research Area(s)

  • Antimicrobial resistance, Minimal inhibitory concentration, Mobile genetic element, Spirochaetes, Swine dysentery, Whole genome sequencing

Citation Format(s)

Transposon-associated lincosamide resistance lnu (C) gene identified in Brachyspira hyodysenteriae ST83. / De Luca, Silvio; Nicholson, Pamela; Magistrali, Chiara F.; García-Martín, Ana B.; Rychener, Lorenz; Zeeh, Friederike; Frey, Joachim; Perreten, Vincent.

In: Veterinary Microbiology, Vol. 214, 02.2018, p. 51-55.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review