Transcriptomic Assay of CD8+ T Cells in Treatment-Naïve HIV, HCV-Mono-Infected and HIV/HCV-Co-Infected Chinese

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Jin Zhao
  • Lina Yi
  • Jing Lu
  • Zheng-Rong Yang
  • Ying Chen
  • Chenli Zheng
  • Dan Huang
  • Yu-Feng Li
  • Lin Chen
  • Jinquan Cheng
  • Hsiang-fu Kung

Detail(s)

Original languageEnglish
Article numbere45200
Journal / PublicationPLoS ONE
Volume7
Issue number9
Publication statusPublished - 13 Sep 2012
Externally publishedYes

Abstract

Background: Co-infection with HIV and HCV is very common. It is estimated that over 5 million people are co-infected with HIV and HCV worldwide. Accumulated evidence shows that each virus alters the course of infection of the other one. CD8+ T cells play a crucial role in the eradication of viruses and infected target cells. To the best of our knowledge, no one has investigated the gene expression profiles in HIV/HCV-co-infected individuals. Methodology: Genome-wide transcriptomes of CD8+ T cells from HIV/HCV-co-infected or mono-infected treatment-naïve individuals were analyzed by microarray assays. Pairwise comparisons were performed and differentially expressed genes were identified followed by quantitative real-time PCR (qRT-PCR) validation. Directed Acyclic Graphs (DAG) from Web-based Gene SeT AnaLysis Toolkit (WebGestalt) and DAVID bioinformatics resources 6.7 (the Database for Annotation, Visualization, and Integrated Discovery) were used to discover the Gene Ontology (GO) categories with significantly enriched gene numbers. The enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were also obtained by using WebGestalt software. Results and Conclusions: A total of 110, 24 and 72 transcript IDs were shown to be differentially expressed (> 2-fold and p

Citation Format(s)

Transcriptomic Assay of CD8+ T Cells in Treatment-Naïve HIV, HCV-Mono-Infected and HIV/HCV-Co-Infected Chinese. / Zhao, Jin; Yi, Lina; Lu, Jing; Yang, Zheng-Rong; Chen, Ying; Zheng, Chenli; Huang, Dan; Li, Yu-Feng; Chen, Lin; Cheng, Jinquan; Kung, Hsiang-fu; He, Ming-Liang.

In: PLoS ONE, Vol. 7, No. 9, e45200, 13.09.2012.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal