Transcriptome analysis of Atlantic salmon (Salmo salar) skin in response to sea lice and infectious salmon anemia virus co-infection under different experimental functional diets

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

11 Scopus Citations
View graph of relations

Author(s)

  • Surendra Kumar
  • Umasuthan Navaneethaiyer
  • Albert Caballero-Solares
  • Laura A. Carvalho
  • Shona K. Whyte
  • Sara L. Purcell
  • Nellie Gagne
  • Tiago S. Hori
  • Melissa Allensworth
  • Richard G. Taylor
  • Rachel Balder
  • Christopher C. Parrish
  • Matthew L. Rise
  • Mark D. Fast

Detail(s)

Original languageEnglish
Article number787033
Journal / PublicationFrontiers in Immunology
Volume12
Online published3 Jan 2022
Publication statusPublished - Jan 2022

Link(s)

Abstract

Sea lice (Lepeophtheirus salmonis) are ectoparasitic copepods that cause significant economic loss in marine salmoniculture. In commercial salmon farms, infestation with sea lice can enhance susceptibility to other significant pathogens, such as the highly contagious infectious salmon anemia virus (ISAv). In this study, transcriptomic analysis was used to evaluate the impact of four experimental functional feeds (i.e. 0.3% EPA/DHA+high-ω6, 0.3% EPA/DHA+high-ω6+immunostimulant (IS), 1% EPA/DHA+high-ω6, and 1% EPA/DHA+high-ω3) on Atlantic salmon (Salmo salar) during a single infection with sea lice (L. salmonis) and a co-infection with sea lice and ISAv. The overall objectives were to compare the transcriptomic profiles of skin between lice infection alone with co-infection groups and assess differences in gene expression response among animals with different experimental diets. Atlantic salmon smolts were challenged with L. salmonis following a 28-day feeding trial. Fish were then challenged with ISAv at 18 days post-sea lice infection (dpi), and maintained on individual diets, to establish a co-infection model. Skin tissues sampled at 33 dpi were subjected to RNA-seq analysis. The co-infection’s overall survival rates were between 37%-50%, while no mortality was observed in the single infection with lice. With regard to the infection status, 756 and 1303 consensus differentially expressed genes (DEGs) among the four diets were identified in “lice infection vs. pre-infection” and “co-infection vs. pre-infection” groups, respectively, that were shared between the four experimental diets. The co-infection groups (co-infection vs. pre-infection) included up-regulated genes associated with glycolysis, the interferon pathway, complement cascade activity, and heat shock protein family, while the down-regulated genes were related to antigen presentation and processing, T-cell activation, collagen formation, and extracellular matrix. Pathway enrichment analysis conducted between infected groups (lice infection vs. co-infection) resulted in several immune-related significant GO terms and pathways unique to this group, such as “autophagosome”, “cytosolic DNA-sensing pathway” and “response to type I interferons”. Understanding how experimental functional feeds can impact the host response and the trajectory of co-infections will be an essential step in identifying efficacious intervention strategies that account for the complexities of disease in open cage culture.

Research Area(s)

  • Atlantic salmon, sea lice, infectious salmon anemia virus (ISAv), co-infection, transcriptome, functional diets, immune response

Citation Format(s)

Transcriptome analysis of Atlantic salmon (Salmo salar) skin in response to sea lice and infectious salmon anemia virus co-infection under different experimental functional diets. / Cai, Wenlong; Kumar, Surendra; Navaneethaiyer, Umasuthan et al.
In: Frontiers in Immunology, Vol. 12, 787033, 01.2022.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

Download Statistics

No data available