Tracking the activity of the Anammox-DAMO process using excitation-emission matrix (EEM) fluorescence spectroscopy

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

9 Scopus Citations
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Author(s)

  • Yong-Ze Lu
  • Na Li
  • Zhao-Wei Ding
  • Liang Fu
  • Guo-Ping Sheng
  • Raymond J. Zeng

Detail(s)

Original languageEnglish
Pages (from-to)624-632
Journal / PublicationWater Research
Volume122
Early online date14 Jun 2017
Publication statusPublished - 1 Oct 2017

Abstract

Coupling of anaerobic ammonium oxidation (anammox) and denitrifying anaerobic methane oxidation (DAMO) microorganisms in a hollow-fiber membrane biofilm reactor (HfMBR) is a potential strategy for simultaneous anaerobic removal of nitrogen and methane in wastewater streams. In these systems, effluents contain dissolved organic substances from anammox and DAMO microorganisms, but their characteristics and relationships have not been investigated. In the present study, excitation-emission matrix (EEM) fluorescence spectroscopy was used to characterize effluent dissolved organic matter (EfDOM) from an Anammox-DAMO HfMBR. Four component types (Component 1-4) were identified by parallel factor analysis (PARAFAC) of EEM data. Component 1 was produced when anammox and DAMO microorganisms simultaneously starved, whereas Component 4 was only generated through the starving period of DAMO microorganisms, and the longer the starving period, the higher the fluorescence intensity of the components. Components 2 and 3 were generated via active and starving periods of co-cultures. More efficient nitrogen removal was accompanied by a higher fluorescence intensity and microbial activity. Compared to measuring both influent and effluent nitrogen concentrations, monitoring EfDOM can obtain other information about the reactor, such as nitrogen removal activity of the reactor, status of the microbes and the duration of starving period the reactor suffered, which therefore offers a complementary but direct tool for assessing reactor performance in complex co-culture systems.

Research Area(s)

  • Anaerobic ammonium oxidation (anammox), Denitrifying anaerobic methane oxidation (DAMO), Excitation-emission matrix (EEM) fluorescence spectroscopy, Effluent dissolved organic matter (EfDOM), Parallel factor analysis (PARAFAC), DISSOLVED ORGANIC-MATTER, SOLUBLE MICROBIAL PRODUCTS, EXTRACELLULAR POLYMERIC SUBSTANCES, METHANE-OXIDIZING MICROORGANISMS, MEMBRANE BIOFILM REACTOR, PARALLEL FACTOR-ANALYSIS, WATER-TREATMENT-PLANT, ACTIVATED-SLUDGE, WASTE-WATER, 3-DIMENSIONAL EXCITATION

Citation Format(s)

Tracking the activity of the Anammox-DAMO process using excitation-emission matrix (EEM) fluorescence spectroscopy. / Lu, Yong-Ze; Li, Na; Ding, Zhao-Wei; Fu, Liang; Bai, Ya-Nan; Sheng, Guo-Ping; Zeng, Raymond J.

In: Water Research, Vol. 122, 01.10.2017, p. 624-632.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review