Tracking Recombination Events That Occur in Conjugative Virulence Plasmid p15WZ-82_Vir during the Transmission Process

Xuemei Yang; Lianwei Ye; Edward Wai-Chi Chan; Rong Zhang; Sheng Chen

doi:10.1128/mSystems.00140-20

Tracking Recombination Events That Occur in Conjugative Virulence Plasmid p15WZ-82_Vir during the Transmission Process

Xuemei Yang
, Lianwei Ye
, Edward Wai-Chi Chan
, Rong Zhang
, Sheng Chen^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

17 Citations (Scopus)

50 Downloads (CityUHK Scholars)

Abstract

We recently reported the recovery of a conjugative virulence plasmid, p15WZ-82_Vir, from a clinical Klebsiella variicola strain. In this study, we found that several new plasmid types were generated due to genetic rearrangement. Partial integration of plasmid p15WZ-82_Vir with existing plasmids such as resistance plasmids by different homologous recombination events was observable in three recipient strains. Such recombination events enable the formation of various types of mosaic plasmids simultaneously carrying virulence-encoding and antibiotic resistance-encoding genes as well as genes involved in plasmid conjugation, which promote transmission of various virulence-encoding and resistance-encoding elements among pathogens. Our data also suggest that these conjugative events may play an integral role in the development of novel mosaic plasmids, which is vital for plasmid evolution.

Original language	English
Article number	e00140-20
Journal	mSystems
Volume	5
Issue number	4
Online published	14 Jul 2020
DOIs	https://doi.org/10.1128/mSystems.00140-20
Publication status	Published - Jul 2020

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

Conjugative
Homologous recombination
Hypervirulence plasmid
Klebsiella pneumoniae
Plasmid evolution

Publisher's Copyright Statement

This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/

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10.1128/mSystems.00140-20

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title = "Tracking Recombination Events That Occur in Conjugative Virulence Plasmid p15WZ-82\_Vir during the Transmission Process",

abstract = "We recently reported the recovery of a conjugative virulence plasmid, p15WZ-82\_Vir, from a clinical Klebsiella variicola strain. In this study, we found that several new plasmid types were generated due to genetic rearrangement. Partial integration of plasmid p15WZ-82\_Vir with existing plasmids such as resistance plasmids by different homologous recombination events was observable in three recipient strains. Such recombination events enable the formation of various types of mosaic plasmids simultaneously carrying virulence-encoding and antibiotic resistance-encoding genes as well as genes involved in plasmid conjugation, which promote transmission of various virulence-encoding and resistance-encoding elements among pathogens. Our data also suggest that these conjugative events may play an integral role in the development of novel mosaic plasmids, which is vital for plasmid evolution.",

keywords = "Conjugative, Homologous recombination, Hypervirulence plasmid, Klebsiella pneumoniae, Plasmid evolution, Conjugative, Homologous recombination, Hypervirulence plasmid, Klebsiella pneumoniae, Plasmid evolution, Conjugative, Homologous recombination, Hypervirulence plasmid, Klebsiella pneumoniae, Plasmid evolution",

author = "Xuemei Yang and Lianwei Ye and Chan, \{Edward Wai-Chi\} and Rong Zhang and Sheng Chen",

year = "2020",

month = jul,

doi = "10.1128/mSystems.00140-20",

language = "English",

volume = "5",

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T1 - Tracking Recombination Events That Occur in Conjugative Virulence Plasmid p15WZ-82_Vir during the Transmission Process

AU - Yang, Xuemei

AU - Ye, Lianwei

AU - Chan, Edward Wai-Chi

AU - Zhang, Rong

AU - Chen, Sheng

PY - 2020/7

Y1 - 2020/7

N2 - We recently reported the recovery of a conjugative virulence plasmid, p15WZ-82_Vir, from a clinical Klebsiella variicola strain. In this study, we found that several new plasmid types were generated due to genetic rearrangement. Partial integration of plasmid p15WZ-82_Vir with existing plasmids such as resistance plasmids by different homologous recombination events was observable in three recipient strains. Such recombination events enable the formation of various types of mosaic plasmids simultaneously carrying virulence-encoding and antibiotic resistance-encoding genes as well as genes involved in plasmid conjugation, which promote transmission of various virulence-encoding and resistance-encoding elements among pathogens. Our data also suggest that these conjugative events may play an integral role in the development of novel mosaic plasmids, which is vital for plasmid evolution.

AB - We recently reported the recovery of a conjugative virulence plasmid, p15WZ-82_Vir, from a clinical Klebsiella variicola strain. In this study, we found that several new plasmid types were generated due to genetic rearrangement. Partial integration of plasmid p15WZ-82_Vir with existing plasmids such as resistance plasmids by different homologous recombination events was observable in three recipient strains. Such recombination events enable the formation of various types of mosaic plasmids simultaneously carrying virulence-encoding and antibiotic resistance-encoding genes as well as genes involved in plasmid conjugation, which promote transmission of various virulence-encoding and resistance-encoding elements among pathogens. Our data also suggest that these conjugative events may play an integral role in the development of novel mosaic plasmids, which is vital for plasmid evolution.

KW - Conjugative

KW - Homologous recombination

KW - Hypervirulence plasmid

KW - Klebsiella pneumoniae

KW - Plasmid evolution

KW - Conjugative

KW - Homologous recombination

KW - Hypervirulence plasmid

KW - Klebsiella pneumoniae

KW - Plasmid evolution

KW - Conjugative

KW - Homologous recombination

KW - Hypervirulence plasmid

KW - Klebsiella pneumoniae

KW - Plasmid evolution

UR - https://www.scopus.com/pages/publications/85090458755

UR - https://www.scopus.com/pages/publications/85090458755#tab=impact

U2 - 10.1128/mSystems.00140-20

DO - 10.1128/mSystems.00140-20

M3 - RGC 21 - Publication in refereed journal

C2 - 32665326

SN - 2379-5077

VL - 5

JO - mSystems

JF - mSystems

IS - 4

M1 - e00140-20

ER -

Tracking Recombination Events That Occur in Conjugative Virulence Plasmid p15WZ-82_Vir during the Transmission Process

Abstract

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Research Keywords

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CRF: Development of Novel Inhibitors Targeting the Resistance Mechanisms of Clinical Superbugs

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Tracking Recombination Events That Occur in Conjugative Virulence Plasmid p15WZ-82_Vir during the Transmission Process

Abstract

UN SDGs

Research Keywords

Publisher's Copyright Statement

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CRF: Development of Novel Inhibitors Targeting the Resistance Mechanisms of Clinical Superbugs

Cite this