Toxicogenomic mechanisms of 6-HO-BDE-47, 6-MeO-BDE-47, and BDE-47 in E. coli

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Guanyong Su
  • Xiaowei Zhang
  • Hongling Liu
  • John P. Giesy
  • Paul K. S. Lam
  • Maqsood A. Siddiqui
  • Javed Musarrat
  • Abdulaziz Al-Khedhairy
  • Hongxia Yu

Detail(s)

Original languageEnglish
Pages (from-to)1185-1191
Journal / PublicationEnvironmental Science and Technology
Volume46
Issue number2
Publication statusPublished - 17 Jan 2012

Abstract

Cytotoxicity of 6-HO-BDE-47 and its two analogues, BDE-47 and 6-MeO-BDE-47, and the associated molecular mechanisms were assessed by use of a live cell reporter assay system which contains a library of 1820 modified green fluorescent protein (GFP) expressing promoter reporter vectors constructed from E. coli K12 strains. 6-HO-BDE-47 inhibited growth of E. coli with a 4 h median effect concentration (EC50) of 22.52 ± 2.20 mg/L, but neither BDE-47 nor 6-MeO-BDE-47 were cytotoxic. Thus, 6-HO-BDE-47 might serve as an antibiotic in some living organisms. Exposure to 6-HO-BDE-47 resulted in 65 (fold change >2) or 129 (fold change >1.5) genes being differentially expressed. The no observed transcriptional effect concentration (NOTEC) and median transcriptional effect concentration (TEC50) based on transcriptional end points, of 6-HO-BDE-47 were 0.0438 and 0.580 mg/L, respectively. The transcriptional responses were 514- and 39-fold more sensitive than the acute EC50 to inhibit cell growth. Most of the genes that were differentially expressed in response to 6-HO-BDE-47 were not modulated by BDE-47 or 6-MeO-BDE-47. These results suggest that cytotoxicity of 6-HO-BDE-47 to E. coli was via a mechanism that was different from that of either BDE-47 or 6-MeO-BDE-47. Gene expression associated with metabolic pathways was more responsive to 6-HO-BDE-47, which suggests that this pathway might be the primary target of this compound. © 2011 American Chemical Society.

Citation Format(s)

Toxicogenomic mechanisms of 6-HO-BDE-47, 6-MeO-BDE-47, and BDE-47 in E. coli. / Su, Guanyong; Zhang, Xiaowei; Liu, Hongling; Giesy, John P.; Lam, Michael H. W.; Lam, Paul K. S.; Siddiqui, Maqsood A.; Musarrat, Javed; Al-Khedhairy, Abdulaziz; Yu, Hongxia.

In: Environmental Science and Technology, Vol. 46, No. 2, 17.01.2012, p. 1185-1191.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review