Towards a metagenomic understanding on enhanced biomethane production from waste activated sludge after pH 10 pretreatment

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

95 Scopus Citations
View graph of relations

Author(s)

  • Mabel Ting Wong
  • Dong Zhang
  • Raymond Kin Hi Hui
  • Hein Min Tun
  • Manreetpal Singh Brar
  • Tae-Jin Park
  • Yinguang Chen
  • Frederick C Leung

Detail(s)

Original languageEnglish
Article number38
Journal / PublicationBiotechnology for Biofuels
Volume6
Publication statusPublished - 19 Mar 2013
Externally publishedYes

Link(s)

Abstract

Background: Understanding the effects of pretreatment on anaerobic digestion of sludge waste from wastewater treatment plants is becoming increasingly important, as impetus moves towards the utilization of sludge for renewable energy production. Although the field of sludge pretreatment has progressed significantly over the past decade, critical questions concerning the underlying microbial interactions remain unanswered. In this study, a metagenomic approach was adopted to investigate the microbial composition and gene content contributing to enhanced biogas production from sludge subjected to a novel pretreatment method (maintaining pH at 10 for 8 days) compared to other documented methods (ultrasonic, thermal and thermal-alkaline). 
Results: Our results showed that pretreated sludge attained a maximum methane yield approximately 4-fold higher than that of the blank un-pretreated sludge set-up at day 17. Both the microbial and metabolic consortium shifted extensively towards enhanced biodegradation subsequent to pretreatment, providing insight for the enhanced methane yield. The prevalence of Methanosaeta thermophila and Methanothermobacter thermautotrophicus, together with the functional affiliation of enzymes-encoding genes suggested an acetoclastic and hydrogenotrophic methanogenesis pathway. Additionally, an alternative enzymology in Methanosaeta was observed. 
Conclusions: This study is the first to provide a microbiological understanding of improved biogas production subsequent to a novel waste sludge pretreatment method. The knowledge garnered will assist the design of more efficient pretreatment methods for biogas production in the future.

Research Area(s)

  • Biomethane, Metagenomic, Pretreatment, Pyrosequencing, Renewable energy, Sludge, Wastewater treatment plant

Citation Format(s)

Towards a metagenomic understanding on enhanced biomethane production from waste activated sludge after pH 10 pretreatment. / Wong, Mabel Ting; Zhang, Dong; Li, Jun; Hui, Raymond Kin Hi; Tun, Hein Min; Brar, Manreetpal Singh; Park, Tae-Jin; Chen, Yinguang; Leung, Frederick C.

In: Biotechnology for Biofuels, Vol. 6, 38, 19.03.2013.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

Download Statistics

No data available