Toward flexible regulation of polyhydroxyalkanoate composition based on substrate feeding strategy: Insights into microbial community and metabolic features

Long Huang; Zhiqiang Chen; Qinxue Wen; Ye Ji; Zening Wu; Duu-Jong Lee

doi:10.1016/j.biortech.2019.122369

Toward flexible regulation of polyhydroxyalkanoate composition based on substrate feeding strategy : Insights into microbial community and metabolic features

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

Overview

14 Scopus Citations

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Author(s)

Long Huang
Zhiqiang Chen
Qinxue Wen
Ye Ji
Zening Wu

Detail(s)

Original language	English
Article number	122369
Journal / Publication	Bioresource Technology
Volume	296
Online published	5 Nov 2019
Publication status	Published - Jan 2020
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1016/j.biortech.2019.122369 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-85075329520&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(3b6eb07a-b7b4-4357-97b3-7ccb7aad070e).html

Abstract

The suitable feeding strategy considering both substrate preference (enrichment stage) and flexible regulation (PHA accumulation stage) were investigated, respectively, based on intracellular polymers synthesis peculiarities of the three types of cultures (M-Ac, M-Pr and M-Bu), which were enriched correspondingly using acetic type, propionic type and butyric type substrate. Compared to M-Ac and M-Bu cultures, maximum PHA content (PHA_m) of M-Pr exhibited the most stable responses to varying fractions of propionic acid (f_Pr) of the substrate. The substrate composed of acetic acid and propionic acid (Mix-AP) demonstrated higher efficiency in regulation of polymer composition than that composed of butyric acid and propionic acid (Mix-BP). For the whole process of three-stage MC PHA production, propionic acid-dominated acidification products should be used for the long-term enrichment of PHA producers, and acidification products mainly composed of propionic and acetic acid are preferred considering the regulation of polymer composition in PHA accumulation stage.

Research Area(s)

Community structure, Metabolic flux, Mixed culture (MC), Polyhydroxyalkanoate (PHA), Polymer composition

Citation Format(s)

[ RIS ] [ BibTeX ]

Toward flexible regulation of polyhydroxyalkanoate composition based on substrate feeding strategy: Insights into microbial community and metabolic features. / Huang, Long; Chen, Zhiqiang; Wen, Qinxue et al.
In: Bioresource Technology, Vol. 296, 122369, 01.2020.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

Huang, L, Chen, Z, Wen, Q, Ji, Y, Wu, Z & Lee, D-J 2020, 'Toward flexible regulation of polyhydroxyalkanoate composition based on substrate feeding strategy: Insights into microbial community and metabolic features', Bioresource Technology, vol. 296, 122369. https://doi.org/10.1016/j.biortech.2019.122369

Huang, L., Chen, Z., Wen, Q., Ji, Y., Wu, Z., & Lee, D-J. (2020). Toward flexible regulation of polyhydroxyalkanoate composition based on substrate feeding strategy: Insights into microbial community and metabolic features. Bioresource Technology, 296, [122369]. https://doi.org/10.1016/j.biortech.2019.122369

Huang L, Chen Z, Wen Q, Ji Y, Wu Z, Lee D-J. Toward flexible regulation of polyhydroxyalkanoate composition based on substrate feeding strategy: Insights into microbial community and metabolic features. Bioresource Technology. 2020 Jan;296:122369. Epub 2019 Nov 5. doi: 10.1016/j.biortech.2019.122369

Huang, Long ; Chen, Zhiqiang ; Wen, Qinxue et al. / Toward flexible regulation of polyhydroxyalkanoate composition based on substrate feeding strategy : Insights into microbial community and metabolic features. In: Bioresource Technology. 2020 ; Vol. 296.