Toward a PCR-Independent Molecular Diagnosis of Veterinary and Medically Relevant Pathogenic Organisms

Matthew W. Partington; Dino Masic; Audrey Nicholson; John Perry; Andrew Berrington; Jonatan West; Olivier A. E. Sparagano

doi:10.1196/annals.1428.010

Toward a PCR-Independent Molecular Diagnosis of Veterinary and Medically Relevant Pathogenic Organisms

Matthew W. Partington^*
, Dino Masic
, Audrey Nicholson
, John Perry
, Andrew Berrington
, Jonatan West
, Olivier A. E. Sparagano

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

Bloodstream infections caused by bacteria and fungi are a major problem worldwide. These bloodstream infections can affect both people and livestock, placing a significant burden upon developed and developing economies. In this paper we describe a multiplexed testing format, which can identify a range of bacteria and fungi within a single blood sample. Key to this technique is the specificity and sensitivity of the nucleotide probes that capture the sample. The sensitivity and specificity of the probes may allow detection of disease-causing microorganisms without the need for polymerase chain reaction amplification if the dynamics of probe binding can be observed in real time.

Original language	English
Title of host publication	Animal Biodiversity and Emerging Diseases
Subtitle of host publication	Prediction and Prevention
Editors	Olivier A.E. Sparagano, Jean-Charles Maillard, Julio Vicente Figueroa
Publisher	Wiley-Blackwell
Pages	391-393
ISBN (Print)	9781573317146
DOIs	https://doi.org/10.1196/annals.1428.010
Publication status	Published - Dec 2008
Externally published	Yes
Event	9th Biennial Conference of the International Society for Tropical Veterinary Medicine (STVM 2007) - Merida, Mexico Duration: 17 Jun 2007 → 22 Jun 2007

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1149
ISSN (Print)	0077-8923
ISSN (Electronic)	1749-6632

Conference

Conference	9th Biennial Conference of the International Society for Tropical Veterinary Medicine (STVM 2007)
Abbreviated title	STVM 2007
Place	Mexico
City	Merida
Period	17/06/07 → 22/06/07

Research Keywords

Aspergillus
Candida
Diagnostics
PCR
Reverse line blot

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10.1196/annals.1428.010

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Partington, M. W., Masic, D., Nicholson, A., Perry, J., Berrington, A., West, J., & Sparagano, O. A. E. (2008). Toward a PCR-Independent Molecular Diagnosis of Veterinary and Medically Relevant Pathogenic Organisms. In O. A. E. Sparagano, J.-C. Maillard, & J. V. Figueroa (Eds.), Animal Biodiversity and Emerging Diseases: Prediction and Prevention (pp. 391-393). (Annals of the New York Academy of Sciences; Vol. 1149). Wiley-Blackwell. https://doi.org/10.1196/annals.1428.010

Partington, Matthew W. ; Masic, Dino ; Nicholson, Audrey et al. / Toward a PCR-Independent Molecular Diagnosis of Veterinary and Medically Relevant Pathogenic Organisms. Animal Biodiversity and Emerging Diseases: Prediction and Prevention. editor / Olivier A.E. Sparagano ; Jean-Charles Maillard ; Julio Vicente Figueroa. Wiley-Blackwell, 2008. pp. 391-393 (Annals of the New York Academy of Sciences).

@inproceedings{fa3de04a2a3d4aca91406edbf4cc1b11,

title = "Toward a PCR-Independent Molecular Diagnosis of Veterinary and Medically Relevant Pathogenic Organisms",

abstract = "Bloodstream infections caused by bacteria and fungi are a major problem worldwide. These bloodstream infections can affect both people and livestock, placing a significant burden upon developed and developing economies. In this paper we describe a multiplexed testing format, which can identify a range of bacteria and fungi within a single blood sample. Key to this technique is the specificity and sensitivity of the nucleotide probes that capture the sample. The sensitivity and specificity of the probes may allow detection of disease-causing microorganisms without the need for polymerase chain reaction amplification if the dynamics of probe binding can be observed in real time. ",

keywords = "Aspergillus, Candida, Diagnostics, PCR, Reverse line blot",

author = "Partington, \{Matthew W.\} and Dino Masic and Audrey Nicholson and John Perry and Andrew Berrington and Jonatan West and Sparagano, \{Olivier A. E.\}",

year = "2008",

month = dec,

doi = "10.1196/annals.1428.010",

language = "English",

isbn = "9781573317146",

series = "Annals of the New York Academy of Sciences",

publisher = "Wiley-Blackwell",

pages = "391--393",

editor = "Sparagano, \{Olivier A.E.\} and Jean-Charles Maillard and Figueroa, \{Julio Vicente\}",

booktitle = "Animal Biodiversity and Emerging Diseases",

address = "United States",

note = "9th Biennial Conference of the International Society for Tropical Veterinary Medicine (STVM 2007), STVM 2007 ; Conference date: 17-06-2007 Through 22-06-2007",

}

Partington, MW, Masic, D, Nicholson, A, Perry, J, Berrington, A, West, J & Sparagano, OAE 2008, Toward a PCR-Independent Molecular Diagnosis of Veterinary and Medically Relevant Pathogenic Organisms. in OAE Sparagano, J-C Maillard & JV Figueroa (eds), Animal Biodiversity and Emerging Diseases: Prediction and Prevention. Annals of the New York Academy of Sciences, vol. 1149, Wiley-Blackwell, pp. 391-393, 9th Biennial Conference of the International Society for Tropical Veterinary Medicine (STVM 2007), Merida, Mexico, 17/06/07. https://doi.org/10.1196/annals.1428.010

Toward a PCR-Independent Molecular Diagnosis of Veterinary and Medically Relevant Pathogenic Organisms. / Partington, Matthew W.; Masic, Dino; Nicholson, Audrey et al.
Animal Biodiversity and Emerging Diseases: Prediction and Prevention. ed. / Olivier A.E. Sparagano; Jean-Charles Maillard; Julio Vicente Figueroa. Wiley-Blackwell, 2008. p. 391-393 (Annals of the New York Academy of Sciences; Vol. 1149).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Toward a PCR-Independent Molecular Diagnosis of Veterinary and Medically Relevant Pathogenic Organisms

AU - Partington, Matthew W.

AU - Masic, Dino

AU - Nicholson, Audrey

AU - Perry, John

AU - Berrington, Andrew

AU - West, Jonatan

AU - Sparagano, Olivier A. E.

PY - 2008/12

Y1 - 2008/12

N2 - Bloodstream infections caused by bacteria and fungi are a major problem worldwide. These bloodstream infections can affect both people and livestock, placing a significant burden upon developed and developing economies. In this paper we describe a multiplexed testing format, which can identify a range of bacteria and fungi within a single blood sample. Key to this technique is the specificity and sensitivity of the nucleotide probes that capture the sample. The sensitivity and specificity of the probes may allow detection of disease-causing microorganisms without the need for polymerase chain reaction amplification if the dynamics of probe binding can be observed in real time.

AB - Bloodstream infections caused by bacteria and fungi are a major problem worldwide. These bloodstream infections can affect both people and livestock, placing a significant burden upon developed and developing economies. In this paper we describe a multiplexed testing format, which can identify a range of bacteria and fungi within a single blood sample. Key to this technique is the specificity and sensitivity of the nucleotide probes that capture the sample. The sensitivity and specificity of the probes may allow detection of disease-causing microorganisms without the need for polymerase chain reaction amplification if the dynamics of probe binding can be observed in real time.

KW - Aspergillus

KW - Candida

KW - Diagnostics

KW - PCR

KW - Reverse line blot

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U2 - 10.1196/annals.1428.010

DO - 10.1196/annals.1428.010

M3 - RGC 32 - Refereed conference paper (with host publication)

C2 - 19120258

SN - 9781573317146

T3 - Annals of the New York Academy of Sciences

SP - 391

EP - 393

BT - Animal Biodiversity and Emerging Diseases

A2 - Sparagano, Olivier A.E.

A2 - Maillard, Jean-Charles

A2 - Figueroa, Julio Vicente

PB - Wiley-Blackwell

T2 - 9th Biennial Conference of the International Society for Tropical Veterinary Medicine (STVM 2007)

Y2 - 17 June 2007 through 22 June 2007

ER -

Partington MW, Masic D, Nicholson A, Perry J, Berrington A, West J et al. Toward a PCR-Independent Molecular Diagnosis of Veterinary and Medically Relevant Pathogenic Organisms. In Sparagano OAE, Maillard JC, Figueroa JV, editors, Animal Biodiversity and Emerging Diseases: Prediction and Prevention. Wiley-Blackwell. 2008. p. 391-393. (Annals of the New York Academy of Sciences). Epub 2008 Dec 15. doi: 10.1196/annals.1428.010

Toward a PCR-Independent Molecular Diagnosis of Veterinary and Medically Relevant Pathogenic Organisms

Abstract

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Research Keywords

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