Total flavonoids of Herba Epimedii improves osteogenesis and inhibits osteoclastogenesis of human mesenchymal stem cells

Jin-Fang Zhang; Guo Li; Chun-Ling Meng; Qi Dong; Chu-Yan Chan; Ming-Liang He; Ping-Chung Leung; Ya-Ou Zhang; Hsiang-Fu Kung

doi:10.1016/j.phymed.2009.01.003

Author(s)

Jin-Fang Zhang
Guo Li
Chun-Ling Meng
Qi Dong
Chu-Yan Chan
Ping-Chung Leung
Ya-Ou Zhang
Hsiang-Fu Kung

Detail(s)

Original language	English
Pages (from-to)	521-529
Journal / Publication	Phytomedicine
Volume	16
Issue number	6-7
Publication status	Published - Jun 2009
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1016/j.phymed.2009.01.003 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-67349095370&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(00f636f1-39f3-4f76-af0c-7f0fa39f1cd8).html

Abstract

Introduction: In China Herba Epimedii is one of the most common herbs that could be prescribed for treating osteoporosis. It is known to increase the overall mineral content, therefore, to promote bone formation and to increase lumbar bone mineral density (BMD). The present study was aimed at investigating the effect of flavonoids of Herba Epimedii (HEF) on osteogenesis in human MSCs. Methods: The human bone marrow-derived MSCs (BM-MSCs) were isolated and their osteogenic differentiation was evaluated by their alkaline phosphatase (ALP) activities and level of mineralization. After treating with total flavonoids during osteogenic differentiation process, differential mRNA expression was examined by RT-PCR. Results: The total time needed for osteogenic differentiation of BM-MSCs was significantly shortened by adding HEF. Up-regulation of mRNA expression by HEF was observed for several marker genes and osteogenic regulators. HEF was also found to inhibit osteoclastogenesis of MSCs by enhancing the ratio OPG/RANKL. Conclusions: Our study demonstrated that the HEF could improve osteogenic differentiation and inhibit the osteoclast differentiation of BM-MSCs concurrently. © 2009.

Research Area(s)

Bone marrow, Flavonoids of Herba Epimedii, Mesenchymal stem cells, Osteoclastogenesis, Osteogenesis

Citation Format(s)

[ RIS ] [ BibTeX ]

Total flavonoids of Herba Epimedii improves osteogenesis and inhibits osteoclastogenesis of human mesenchymal stem cells. / Zhang, Jin-Fang; Li, Guo; Meng, Chun-Ling et al.
In: Phytomedicine, Vol. 16, No. 6-7, 06.2009, p. 521-529.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Zhang, J-F, Li, G, Meng, C-L, Dong, Q, Chan, C-Y, He, M-L, Leung, P-C, Zhang, Y-O & Kung, H-F 2009, 'Total flavonoids of Herba Epimedii improves osteogenesis and inhibits osteoclastogenesis of human mesenchymal stem cells', Phytomedicine, vol. 16, no. 6-7, pp. 521-529. https://doi.org/10.1016/j.phymed.2009.01.003

Zhang, J.-F., Li, G., Meng, C.-L., Dong, Q., Chan, C.-Y., He, M.-L., Leung, P.-C., Zhang, Y.-O., & Kung, H.-F. (2009). Total flavonoids of Herba Epimedii improves osteogenesis and inhibits osteoclastogenesis of human mesenchymal stem cells. Phytomedicine, 16(6-7), 521-529. https://doi.org/10.1016/j.phymed.2009.01.003

Zhang JF, Li G, Meng CL, Dong Q, Chan CY, He ML et al. Total flavonoids of Herba Epimedii improves osteogenesis and inhibits osteoclastogenesis of human mesenchymal stem cells. Phytomedicine. 2009 Jun;16(6-7):521-529. doi: 10.1016/j.phymed.2009.01.003

Zhang, Jin-Fang ; Li, Guo ; Meng, Chun-Ling et al. / Total flavonoids of Herba Epimedii improves osteogenesis and inhibits osteoclastogenesis of human mesenchymal stem cells. In: Phytomedicine. 2009 ; Vol. 16, No. 6-7. pp. 521-529.