Tissue morphology controlled by micropatterning and self-assembly of vascular mesenchymal cells

Ting-Hsuan Chen; Leiting Pan; Xiaolu Zhu; Chih-Ming Ho

doi:10.1109/NEMS.2013.6559743

Tissue morphology controlled by micropatterning and self-assembly of vascular mesenchymal cells

Ting-Hsuan Chen^*
, Leiting Pan
, Xiaolu Zhu
, Chih-Ming Ho

^*Corresponding author for this work

School of Creative Media

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

1 Citation (Scopus)

Abstract

Creating patterns of constituent ingredients are essential for tissue regeneration. Using vascular mesenchymal cells (VMCs) which spontaneously aggregate into multicellular structure, we demonstrate a MEMS-based method to direct the assembly of VMCs into desired tissue patterns. Incorporating the inherent chirality of VMCs revealed by micro-engineered substrates, differences in initial cell plating can be amplified into the formation of exquisite radial structures resembling the cross-sectional structure of liver lobules. Furthermore, when co-cultured with VMCs, vascular endothelial cells (ECs) tracked with the VMCs and formed a coherent radial pattern, indicating the applicability to heterotypical cell organization. We envision method has broad implications for building instructive microenvironment for tissue engineering. © 2013 IEEE.

Original language	English
Title of host publication	The 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS2013)
Publisher	IEEE
Pages	328-331
ISBN (Electronic)	9781467363525, 978-1-4673-6351-8
DOIs	https://doi.org/10.1109/NEMS.2013.6559743
Publication status	Published - 2013
Event	8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS 2013) - Suzhou, China Duration: 7 Apr 2013 → 10 Apr 2013 http://cnm.cust.edu.cn/en/node/26

Conference

Conference	8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS 2013)
Abbreviated title	NEMS 2013
Place	China
City	Suzhou
Period	7/04/13 → 10/04/13
Internet address	http://cnm.cust.edu.cn/en/node/26

Research Keywords

micropatterning
self-assembly
tissue morphogenesis

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10.1109/NEMS.2013.6559743

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title = "Tissue morphology controlled by micropatterning and self-assembly of vascular mesenchymal cells",

abstract = "Creating patterns of constituent ingredients are essential for tissue regeneration. Using vascular mesenchymal cells (VMCs) which spontaneously aggregate into multicellular structure, we demonstrate a MEMS-based method to direct the assembly of VMCs into desired tissue patterns. Incorporating the inherent chirality of VMCs revealed by micro-engineered substrates, differences in initial cell plating can be amplified into the formation of exquisite radial structures resembling the cross-sectional structure of liver lobules. Furthermore, when co-cultured with VMCs, vascular endothelial cells (ECs) tracked with the VMCs and formed a coherent radial pattern, indicating the applicability to heterotypical cell organization. We envision method has broad implications for building instructive microenvironment for tissue engineering. {\textcopyright} 2013 IEEE.",

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author = "Ting-Hsuan Chen and Leiting Pan and Xiaolu Zhu and Chih-Ming Ho",

year = "2013",

doi = "10.1109/NEMS.2013.6559743",

language = "English",

pages = "328--331",

booktitle = "The 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS2013)",

publisher = "IEEE",

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note = "8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS 2013), NEMS 2013 ; Conference date: 07-04-2013 Through 10-04-2013",

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Chen, T-H, Pan, L, Zhu, X & Ho, C-M 2013, Tissue morphology controlled by micropatterning and self-assembly of vascular mesenchymal cells. in The 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS2013). IEEE, pp. 328-331, 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS 2013), Suzhou, China, 7/04/13. https://doi.org/10.1109/NEMS.2013.6559743

Tissue morphology controlled by micropatterning and self-assembly of vascular mesenchymal cells. / Chen, Ting-Hsuan; Pan, Leiting; Zhu, Xiaolu et al.
The 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS2013). IEEE, 2013. p. 328-331.

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Tissue morphology controlled by micropatterning and self-assembly of vascular mesenchymal cells

AU - Chen, Ting-Hsuan

AU - Pan, Leiting

AU - Zhu, Xiaolu

AU - Ho, Chih-Ming

PY - 2013

Y1 - 2013

N2 - Creating patterns of constituent ingredients are essential for tissue regeneration. Using vascular mesenchymal cells (VMCs) which spontaneously aggregate into multicellular structure, we demonstrate a MEMS-based method to direct the assembly of VMCs into desired tissue patterns. Incorporating the inherent chirality of VMCs revealed by micro-engineered substrates, differences in initial cell plating can be amplified into the formation of exquisite radial structures resembling the cross-sectional structure of liver lobules. Furthermore, when co-cultured with VMCs, vascular endothelial cells (ECs) tracked with the VMCs and formed a coherent radial pattern, indicating the applicability to heterotypical cell organization. We envision method has broad implications for building instructive microenvironment for tissue engineering. © 2013 IEEE.

AB - Creating patterns of constituent ingredients are essential for tissue regeneration. Using vascular mesenchymal cells (VMCs) which spontaneously aggregate into multicellular structure, we demonstrate a MEMS-based method to direct the assembly of VMCs into desired tissue patterns. Incorporating the inherent chirality of VMCs revealed by micro-engineered substrates, differences in initial cell plating can be amplified into the formation of exquisite radial structures resembling the cross-sectional structure of liver lobules. Furthermore, when co-cultured with VMCs, vascular endothelial cells (ECs) tracked with the VMCs and formed a coherent radial pattern, indicating the applicability to heterotypical cell organization. We envision method has broad implications for building instructive microenvironment for tissue engineering. © 2013 IEEE.

KW - micropatterning

KW - self-assembly

KW - tissue morphogenesis

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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84883100138&origin=recordpage

U2 - 10.1109/NEMS.2013.6559743

DO - 10.1109/NEMS.2013.6559743

M3 - RGC 32 - Refereed conference paper (with host publication)

SP - 328

EP - 331

BT - The 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS2013)

PB - IEEE

T2 - 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS 2013)

Y2 - 7 April 2013 through 10 April 2013

ER -

Tissue morphology controlled by micropatterning and self-assembly of vascular mesenchymal cells

Abstract

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Research Keywords

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