Tissue iron is negatively correlated with TERC or TERT mRNA expression: A heterochronic parabiosis study in mice

Meng-Wan Zhang; Peng Zhao; Wing-Ho Yung; Yuan Sheng; Ya Ke; Zhong-Ming Qian

doi:10.18632/aging.101676

Tissue iron is negatively correlated with TERC or TERT mRNA expression: A heterochronic parabiosis study in mice

Meng-Wan Zhang, Peng Zhao, Wing-Ho Yung, Yuan Sheng, Ya Ke^*, Zhong-Ming Qian

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

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Abstract

To test the hypothesis that iron accumulation in tissues with age is a key harmful factor for the development of aging, we established heterochronic parabiosis-pairings and investigated changes in serum iron, the expression of major iron transport proteins and iron contents, as well as telomerase reverse transcriptase (TERT), telomerase RNA component (TERC), and telomere length in the liver, kidney and heart of Y-O(O) (old pairing with young), Y-O(Y) (young pairing with old), O-O (pairings between two old) and Y-Y (pairings between two young) mice. We demonstrated that the reduced serum iron, increased iron and reduced expression of TERT and TERC in the tissues of aged mice are reversible by exposure to a younger mouse's circulation. All of these measurements in young mice are reversible by exposure to an older mouse's circulation. Correlation analysis showed that tissue iron is negatively correlated with TERT and TERC expression in the liver, kidney and heart of parabiotic mice. These findings provide new evidence for the key role of iron in aging and also imply the existence of rejuvenating factors in young serum with an anti-ageing role that act by reversing the impaired activity of iron metabolism in old mice. © Zhang et al.

Original language	English
Pages (from-to)	3834-3850
Journal	Aging
Volume	10
Issue number	12
DOIs	https://doi.org/10.18632/aging.101676
Publication status	Published - 1 Dec 2018
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

The studies were supported by the National Natural Science Foundation of China (NSFC31330035, NSFC31571195), the Competitive Earmarked Grants of the Hong Kong Research Grants Council (GRF14111815, GRF 14167817, GRF14107616), the Hong Kong Health & Medical Research Fund (HMRF: 03141436-KY), the Hong Kong Innovation and Technology Fund of (ITS/163/16), and the National 973 Programs (2014CB541604).

Research Keywords

Heterochronic parabiosis
Iron homeostasis
Kidney and heart of mice
Liver
Telomere and telomerase
Young and old mice

Publisher's Copyright Statement

This full text is made available under CC-BY 3.0. https://creativecommons.org/licenses/by/3.0/

RGC Funding Information

RGC-funded

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abstract = "To test the hypothesis that iron accumulation in tissues with age is a key harmful factor for the development of aging, we established heterochronic parabiosis-pairings and investigated changes in serum iron, the expression of major iron transport proteins and iron contents, as well as telomerase reverse transcriptase (TERT), telomerase RNA component (TERC), and telomere length in the liver, kidney and heart of Y-O(O) (old pairing with young), Y-O(Y) (young pairing with old), O-O (pairings between two old) and Y-Y (pairings between two young) mice. We demonstrated that the reduced serum iron, increased iron and reduced expression of TERT and TERC in the tissues of aged mice are reversible by exposure to a younger mouse's circulation. All of these measurements in young mice are reversible by exposure to an older mouse's circulation. Correlation analysis showed that tissue iron is negatively correlated with TERT and TERC expression in the liver, kidney and heart of parabiotic mice. These findings provide new evidence for the key role of iron in aging and also imply the existence of rejuvenating factors in young serum with an anti-ageing role that act by reversing the impaired activity of iron metabolism in old mice. {\textcopyright} Zhang et al.",

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Tissue iron is negatively correlated with TERC or TERT mRNA expression: A heterochronic parabiosis study in mice

Abstract

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Research Keywords

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