Time-lapse imaging reveals dynamic relocalization of PP1γ throughout the mammalian cell cycle

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Laura Trinkle-Mulcahy
  • Paul D. Andrews
  • Sasala Wickramasinghe
  • Judith Sleeman
  • Alan Prescott
  • Carol Lyon
  • Jason R. Swedlow
  • Angus I. Lamond

Detail(s)

Original languageEnglish
Pages (from-to)107-117
Journal / PublicationMolecular Biology of the Cell
Volume14
Issue number1
Publication statusPublished - 1 Jan 2003
Externally publishedYes

Abstract

Protein phosphatase 1 (PP1) is a ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. When transiently expressed as fluorescent protein (FP) fusions, the three PP1 isoforms, α, β/δ, and γ1, are active phosphatases with distinct localization patterns. We report here the establishment and characterization of HeLa cell lines stably expressing either FP-PP1γ or FP alone. Time-lapse imaging reveals dynamic targeting of FP-PP1γ to specific sites throughout the cell cycle, contrasting with the diffuse pattern observed for FP alone. FP-PP1γ shows a nucleolar accumulation during interphase. On entry into mitosis, it localizes initially at kinetochores, where it exchanges rapidly with the diffuse cytoplasmic pool. A dramatic relocalization of PP1 to the chromosome-containing regions occurs at the transition from early to late anaphase, and by telophase FP-PP1γ also accumulates at the cleavage furrow and midbody. The changing spatio-temporal distribution of PP1γ revealed using the stable PP1 cell lines implicates it in multiple processes, including nucleolar function, the regulation of chromosome segregation and cytokinesis.

Citation Format(s)

Time-lapse imaging reveals dynamic relocalization of PP1γ throughout the mammalian cell cycle. / Trinkle-Mulcahy, Laura; Andrews, Paul D.; Wickramasinghe, Sasala; Sleeman, Judith; Prescott, Alan; Lam, Yun Wah; Lyon, Carol; Swedlow, Jason R.; Lamond, Angus I.

In: Molecular Biology of the Cell, Vol. 14, No. 1, 01.01.2003, p. 107-117.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review