Time-Resolved Luminescent Sensing and Imaging for Enzyme Catalytic Activity Based on Responsive Probes

Jiawei Wang; Yibiao Jin; Mingdang Li; Shujuan Liu; Kenneth Kam-Wing Lo; Qiang Zhao

doi:10.1002/asia.202200429

Time-Resolved Luminescent Sensing and Imaging for Enzyme Catalytic Activity Based on Responsive Probes

Jiawei Wang (Co-first Author), Yibiao Jin (Co-first Author), Mingdang Li, Shujuan Liu^*, Kenneth Kam-Wing Lo^*, Qiang Zhao^*

^*Corresponding author for this work

Department of Chemistry

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

6 Citations (Scopus)

76 Downloads (CityUHK Scholars)

Abstract

Enzymes, as a kind of biomacromolecules, play an important role in many physiological processes and relate directly to various diseases. Developing an efficient detection method for enzyme activity is important to achieve early diagnosis of enzyme-relevant diseases and high throughput screening of potential enzyme-relevant drugs. Time-resolved luminescence assay provide a high accuracy and signal-to-noise ratios detection methods for enzyme activity, which has been widely used in high throughput screening of enzyme-relevant drugs and diagnosis of enzyme-relevant diseases. Inspired by these advantages, various responsive probes based on metal complexes and metal-free organic compounds have been developed for time-resolved bioimaging and biosensing of enzyme activity owing to their long luminescence lifetimes, high quantum yields and photostability. In this review, we comprehensively reviewed metal complex- and metal-free organic compound-based responsive probes applied to detect enzyme activity through time-resolved imaging, including their design strategies and sensing principles. Current challenges and future prospects in this rapidly growing field are also discussed.

Original language	English
Article number	e202200429
Journal	Chemistry - An Asian Journal
Volume	17
Issue number	16
Online published	12 Jul 2022
DOIs	https://doi.org/10.1002/asia.202200429
Publication status	Published - 15 Aug 2022

Funding

The authors acknowledge financial support from the National Natural Science Foundation of China (22075148 and 22161160318), National Key Research and Development Program of China (2017YFA0205302) and the National Natural Science Foundation of China/Hong Kong Research Grants Council (N_CityU104/21)

Research Keywords

enzyme
metal complexes
metal-free organic compounds
responsive probes
time-resolved

Publisher's Copyright Statement

COPYRIGHT TERMS OF DEPOSITED POSTPRINT FILE: This is the peer reviewed version of the following article: Wang, J., Jin, Y., Li, M., Liu, S., Lo, K. K-W., & Zhao, Q. (2022). Time-Resolved Luminescent Sensing and Imaging for Enzyme Catalytic Activity Based on Responsive Probes. Chemistry - An Asian Journal, 17(16), [e202200429], which has been published in final form at https://doi.org/10.1002/asia.202200429.
This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.

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title = "Time-Resolved Luminescent Sensing and Imaging for Enzyme Catalytic Activity Based on Responsive Probes",

abstract = "Enzymes, as a kind of biomacromolecules, play an important role in many physiological processes and relate directly to various diseases. Developing an efficient detection method for enzyme activity is important to achieve early diagnosis of enzyme-relevant diseases and high throughput screening of potential enzyme-relevant drugs. Time-resolved luminescence assay provide a high accuracy and signal-to-noise ratios detection methods for enzyme activity, which has been widely used in high throughput screening of enzyme-relevant drugs and diagnosis of enzyme-relevant diseases. Inspired by these advantages, various responsive probes based on metal complexes and metal-free organic compounds have been developed for time-resolved bioimaging and biosensing of enzyme activity owing to their long luminescence lifetimes, high quantum yields and photostability. In this review, we comprehensively reviewed metal complex- and metal-free organic compound-based responsive probes applied to detect enzyme activity through time-resolved imaging, including their design strategies and sensing principles. Current challenges and future prospects in this rapidly growing field are also discussed.",

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author = "Jiawei Wang and Yibiao Jin and Mingdang Li and Shujuan Liu and {Kam-Wing Lo}, Kenneth and Qiang Zhao",

year = "2022",

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day = "15",

doi = "10.1002/asia.202200429",

language = "English",

volume = "17",

journal = "Chemistry - An Asian Journal",

issn = "1861-4728",

publisher = "John Wiley & Sons",

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Time-Resolved Luminescent Sensing and Imaging for Enzyme Catalytic Activity Based on Responsive Probes. / Wang, Jiawei (Co-first Author); Jin, Yibiao (Co-first Author); Li, Mingdang et al.
In: Chemistry - An Asian Journal, Vol. 17, No. 16, e202200429, 15.08.2022.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

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T1 - Time-Resolved Luminescent Sensing and Imaging for Enzyme Catalytic Activity Based on Responsive Probes

AU - Wang, Jiawei

AU - Jin, Yibiao

AU - Li, Mingdang

AU - Liu, Shujuan

AU - Kam-Wing Lo, Kenneth

AU - Zhao, Qiang

PY - 2022/8/15

Y1 - 2022/8/15

N2 - Enzymes, as a kind of biomacromolecules, play an important role in many physiological processes and relate directly to various diseases. Developing an efficient detection method for enzyme activity is important to achieve early diagnosis of enzyme-relevant diseases and high throughput screening of potential enzyme-relevant drugs. Time-resolved luminescence assay provide a high accuracy and signal-to-noise ratios detection methods for enzyme activity, which has been widely used in high throughput screening of enzyme-relevant drugs and diagnosis of enzyme-relevant diseases. Inspired by these advantages, various responsive probes based on metal complexes and metal-free organic compounds have been developed for time-resolved bioimaging and biosensing of enzyme activity owing to their long luminescence lifetimes, high quantum yields and photostability. In this review, we comprehensively reviewed metal complex- and metal-free organic compound-based responsive probes applied to detect enzyme activity through time-resolved imaging, including their design strategies and sensing principles. Current challenges and future prospects in this rapidly growing field are also discussed.

AB - Enzymes, as a kind of biomacromolecules, play an important role in many physiological processes and relate directly to various diseases. Developing an efficient detection method for enzyme activity is important to achieve early diagnosis of enzyme-relevant diseases and high throughput screening of potential enzyme-relevant drugs. Time-resolved luminescence assay provide a high accuracy and signal-to-noise ratios detection methods for enzyme activity, which has been widely used in high throughput screening of enzyme-relevant drugs and diagnosis of enzyme-relevant diseases. Inspired by these advantages, various responsive probes based on metal complexes and metal-free organic compounds have been developed for time-resolved bioimaging and biosensing of enzyme activity owing to their long luminescence lifetimes, high quantum yields and photostability. In this review, we comprehensively reviewed metal complex- and metal-free organic compound-based responsive probes applied to detect enzyme activity through time-resolved imaging, including their design strategies and sensing principles. Current challenges and future prospects in this rapidly growing field are also discussed.

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Time-Resolved Luminescent Sensing and Imaging for Enzyme Catalytic Activity Based on Responsive Probes

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NSFC: Design and Preparation of Phosphorescent Lifetime-Responsive Polymeric Probes for Enzyme Targeting and Catalytic Activity Sensing in Living Cells

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Time-Resolved Luminescent Sensing and Imaging for Enzyme Catalytic Activity Based on Responsive Probes

Abstract

Funding

Research Keywords

Publisher's Copyright Statement

RGC Funding Information

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NSFC: Design and Preparation of Phosphorescent Lifetime-Responsive Polymeric Probes for Enzyme Targeting and Catalytic Activity Sensing in Living Cells

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