Three-Dimensional Normal Human Neural Progenitor Tissue-Like Assemblies : A Model of Persistent Varicella-Zoster Virus Infection
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Detail(s)
Original language | English |
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Article number | e1003512 |
Journal / Publication | PLoS Pathogens |
Volume | 9 |
Issue number | 8 |
Online published | 1 Aug 2013 |
Publication status | Published - Aug 2013 |
Externally published | Yes |
Link(s)
DOI | DOI |
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Attachment(s) | Documents
Publisher's Copyright Statement
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Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-84883425255&origin=recordpage |
Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(d15cfc54-d4df-4687-a7e3-14b2fcaf2c89).html |
Abstract
Varicella-zoster virus (VZV) is a neurotropic human alphaherpesvirus that causes varicella upon primary infection, establishes latency in multiple ganglionic neurons, and can reactivate to cause zoster. Live attenuated VZV vaccines are available; however, they can also establish latent infections and reactivate. Studies of VZV latency have been limited to the analyses of human ganglia removed at autopsy, as the virus is strictly a human pathogen. Recently, terminally differentiated human neurons have received much attention as a means to study the interaction between VZV and human neurons; however, the short life-span of these cells in culture has limited their application. Herein, we describe the construction of a model of normal human neural progenitor cells (NHNP) in tissue-like assemblies (TLAs), which can be successfully maintained for at least 180 days in three-dimensional (3D) culture, and exhibit an expression profile similar to that of human trigeminal ganglia. Infection of NHNP TLAs with cell-free VZV resulted in a persistent infection that was maintained for three months, during which the virus genome remained stable. Immediate-early, early and late VZV genes were transcribed, and low-levels of infectious VZV were recurrently detected in the culture supernatant. Our data suggest that NHNP TLAs are an effective system to investigate long-term interactions of VZV with complex assemblies of human neuronal cells.
Research Area(s)
Citation Format(s)
Three-Dimensional Normal Human Neural Progenitor Tissue-Like Assemblies: A Model of Persistent Varicella-Zoster Virus Infection. / Goodwin, Thomas J.; McCarthy, Maureen; Osterrieder, Nikolaus et al.
In: PLoS Pathogens, Vol. 9, No. 8, e1003512, 08.2013.
In: PLoS Pathogens, Vol. 9, No. 8, e1003512, 08.2013.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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