Three-dimensional (3D) hydrogel serves as a platform to identify potential markers of chondrocyte dedifferentiation by combining RNA sequencing

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

1 Scopus Citations
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Author(s)

  • Yang Ling
  • Weiyuan Zhang
  • Peiyan Wang
  • Wanhua Xie
  • Wei Yang
  • Changjiang Fan

Detail(s)

Original languageEnglish
Pages (from-to)2914-2926
Journal / PublicationBioactive Materials
Volume6
Issue number9
Online published23 Feb 2021
Publication statusPublished - Sep 2021

Link(s)

Abstract

Dedifferentiation of chondrocyte greatly restricts its function and application, however, it is poorly understood except a small number of canonical markers. The non-cell-adhesive property endows polysaccharide hydrogel with the ability to maintain chondrocyte phenotype, which can serve as a platform to identify new molecular markers and therapeutic targets of chondrocyte dedifferentiation. In this study, the high-throughput RNA sequencing (RNA-seq) was first performed on articular chondrocytes at primary (P0) and passage 1 (P1) stages to explore the global alteration of gene expression along with chondrocyte dedifferentiation. Significantly, several potential marker genes, such as PFKFB3, KDM6B, had been identified via comparatively analyzing their expression in P0 and P1 chondrocytes as well as in 3D constructs (i.e. chondrocyte-laden alginate hydrogel and HA-MA hydrogel) at both mRNA and protein level. Besides, the changes in cellular morphology and enriched pathway of differentially expressed genes during chondrocyte dedifferentiation was studied in detail. This study developed the use of hydrogel as a platform to investigate chondrocyte dedifferentiation; the results provided new molecular markers and potential therapeutic targets of chondrocyte dedifferentiation.

Research Area(s)

  • Chondrocyte dedifferentiation, Gene expression, Hydrogel, RNA sequencing

Citation Format(s)

Three-dimensional (3D) hydrogel serves as a platform to identify potential markers of chondrocyte dedifferentiation by combining RNA sequencing. / Ling, Yang; Zhang, Weiyuan; Wang, Peiyan; Xie, Wanhua; Yang, Wei; Wang, Dong-An; Fan, Changjiang.

In: Bioactive Materials, Vol. 6, No. 9, 09.2021, p. 2914-2926.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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