Thermophilic xylanase isolated from the Xerophytic- Cereus pterogonus and Opuntia vulgaris plant sp.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

View graph of relations

Author(s)

  • Vikramathithan Jeyaraman
  • Nirmal Kumar Gali
  • Muthuraman Pandurangan
  • Srikumar Kotteazeth

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)304-311
Journal / PublicationInternational Journal of Phytomedicine
Volume2
Issue number3
Publication statusPublished - 2010

Abstract

A thermo stable xylanase was isolated and characterized from the cladodes of crude Cereus pterogonus and Opuntia vulgaris plant species. The enzyme was active in acidic pH 5.0 and temperature optimum for Cereus pterogonus was 60°C and 80°C, where as in the Opuntia vulgaris plant sp showed three temperature isoforms 50°C, 70°C and 90°C respectively. The metal ions 10mM Co2+, Mn2+, 5mM Ni2+ and 1mM Fe2+ showed enzyme activity at 60°C and 80°C for Cereus pterogonus plant sp, where as Opuntia vulgaris plant sp the maximum activity was observed on 50°C, 70°C and 90°C. The crude homogenate of both plant sp showed several protein bands on SDS-PAGE, but two activity bands on the zymogram analysis. The main aim of the present study was to check the xylanase activity on eukaryotic xerophytic plant sp, surprisingly the enzyme activity was observed in Cereus pterogonus and Opuntia vulgaris plant sp. This study may help protein engineers to design more efficient and acidophilic enzymes for use in different biotechnological processes. In future work the enzyme will purify and characterized for various industrial applications.

Citation Format(s)

Thermophilic xylanase isolated from the Xerophytic- Cereus pterogonus and Opuntia vulgaris plant sp. / Jeyaraman, Vikramathithan; Gali, Nirmal Kumar; Pandurangan, Muthuraman; Kotteazeth, Srikumar.

In: International Journal of Phytomedicine, Vol. 2, No. 3, 2010, p. 304-311.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review