The use of folate-PEG-grafted-hybranched-PEI nonviral vector for the inhibition of glioma growth in the rat

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Bing Liang
  • Chu-yan Chan
  • Yang-chao Chen
  • Xiang-Ping Li
  • Yi Li
  • Dexian Zheng
  • Marie C. Lin
  • Hsiang-Fu Kung
  • Xin-Tao Shuai
  • Ying Peng

Detail(s)

Original languageEnglish
Pages (from-to)4014-4020
Journal / PublicationBiomaterials
Volume30
Issue number23-24
Publication statusPublished - Aug 2009
Externally publishedYes

Abstract

Combined treatment using nonviral agent-mediated enzyme/prodrug therapy and immunotherapy had been proposed as a powerful alternative method of cancer therapy. The present study was aimed to evaluate the cytotoxicity in vitro and the therapeutic efficacy in vivo when the cytosine deaminase/5-fluorocytosine (CD/5-FC) and TNF-related apoptosis-inducing ligand (TRAIL) genes were jointly used against rat C6 glioma cells. The potency of the FA-PEG-PEI used as a nonviral vector was tested in the FR-expressed C6 glioma cells and Wistar rats. The C6 glioma cells and animal model were treated by the combined application of FA-PEG-PEI/pCD/5-FC and FA-PEG-PEI/pTRAIL. The antitumor effect was evaluated by survival assays and tumor volume. This study revealed a significant increase of cytotoxicity in vitro following the combined application of FA-PEG-PEI/pCD/5-FC and FA-PEG-PEI/pTRAIL treatments in C6 glioma cells. Animal studies showed a significant growth inhibition of the C6 glioma xenografts using the combined treatment. These results demonstrated that the combined treatment generated additive cytotoxic effect in C6 glioma cells in both in vitro and in vivo conditions, and indicated that such treatment method using both enzyme/prodrug therapy and TRAIL immunotherapy might be a promising therapeutic strategy in treating glioma. © 2009 Elsevier Ltd. All rights reserved.

Research Area(s)

  • Combined gene therapy, Glioma, In vivo gene transfer, Nonviral vector, PEI PEGylation, Tumor targeting

Citation Format(s)

The use of folate-PEG-grafted-hybranched-PEI nonviral vector for the inhibition of glioma growth in the rat. / Liang, Bing; He, Ming-Liang; Chan, Chu-yan; Chen, Yang-chao; Li, Xiang-Ping; Li, Yi; Zheng, Dexian; Lin, Marie C.; Kung, Hsiang-Fu; Shuai, Xin-Tao; Peng, Ying.

In: Biomaterials, Vol. 30, No. 23-24, 08.2009, p. 4014-4020.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal