The role of 44-methylgambierone in ciguatera fish poisoning : Acute toxicity, production by marine microalgae and its potential as a biomarker for Gambierdiscus spp.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • J. Sam Murray
  • Tomohiro Nishimura
  • Sarah C. Finch
  • Lesley L. Rhodes
  • Jonathan Puddick
  • D. Tim Harwood
  • Michaela E. Larsson
  • Martina A. Doblin
  • Priscilla Leung
  • Frode Rise
  • Alistair L. Wilkins
  • Michèle R. Prinsep

Detail(s)

Original languageEnglish
Article number101853
Journal / PublicationHarmful Algae
Volume97
Online published19 Jun 2020
Publication statusPublished - Jul 2020

Link(s)

Abstract

Ciguatera fish poisoning (CFP) is prevalent around the tropical and sub-tropical latitudes of the world and impacts many Pacific island communities intrinsically linked to the reef system for sustenance and trade. While the genus Gambierdiscus has been linked with CFP, it is commonly found on tropical reef systems in microalgal assemblages with other genera of toxin-producing, epiphytic and/or benthic dinoflagellates – Amphidinium, Coolia, Fukuyoa, Ostreopsis and Prorocentrum. Identifying a biomarker compound that can be used for the early detection of Gambierdiscus blooms, specifically in a mixed microalgal community, is paramount in enabling the development of management and mitigation strategies. Following on from the recent structural elucidation of 44-methylgambierone, its potential to contribute to CFP intoxication events and applicability as a biomarker compound for Gambierdiscus spp. was investigated. The acute toxicity of this secondary metabolite was determined by intraperitoneal injection using mice, which showed it to be of low toxicity, with an LD50 between 20 and 38 mg kg−1. The production of 44-methylgambierone by 252 marine microalgal isolates consisting of 90 species from 32 genera across seven classes, was assessed by liquid chromatography-tandem mass spectrometry. It was discovered that the production of this secondary metabolite was ubiquitous to the eight Gambierdiscus species tested, however not all isolates of G. carpenteri, and some species/isolates of Coolia and Fukuyoa.

Research Area(s)

  • Benthic dinoflagellate, Ciguatoxin, Liquid chromatography-tandem mass spectrometry, Maitotoxin-3, Quantitative nuclear magnetic resonance spectroscopy

Citation Format(s)

The role of 44-methylgambierone in ciguatera fish poisoning: Acute toxicity, production by marine microalgae and its potential as a biomarker for Gambierdiscus spp. / Murray, J. Sam; Nishimura, Tomohiro; Finch, Sarah C. et al.
In: Harmful Algae, Vol. 97, 101853, 07.2020.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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