The microfluidic capture of single breast cancer cells for multi-drug resistance assays

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)12_Chapter in an edited book (Author)peer-review

1 Scopus Citations
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Author(s)

  • Karan Parekh
  • Hamide Sharifi
  • Avid Khamenehfar
  • Timothy V. Beischlag
  • Robert T.M. Payer

Detail(s)

Original languageEnglish
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages113-127
Volume628
ISBN (Print)9780128170908
Publication statusPublished - 1 Jan 2019
Externally publishedYes

Publication series

NameMethods in Enzymology
Volume628
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Abstract

Utilizing the microfluidic single-cell technique enables us to study the inhibition of multidrug resistance due to drug efflux on a single triple-negative breast cancer cell. This method examines drug efflux inhibition on a single cell in a microfluidic chip using a conventional optical detection system constructed from an inverted microscope and a microphotometer. More importantly, the integration of single-cell selection, dye and drug loading, and fluorescence measurement for intracellular drug accumulation is all conducted on a single microfluidic chip. By using a microfluidic chip and the adherent nature of the cancer cell lines, a single breast cancer cell could be selected and retained near the cell retention structure in the chip. This enabled us to detect dye accumulation in the MDR breast cells in the presence of cyclosporine A (CsA). CsA and rhodamine 123 (Rh123) were used as the P-glycoprotein (P-gp) inhibitor and fluorescent dye, respectively. Furthermore, Paclitaxel, a commonly known chemotherapeutic used in breast cancer patients, was administered in the presence of both reagents. During the entirety of the experiment fluorescence measurement was used to monitor the fluctuating levels of intracellular Rh123 levels, and an optical imaging system was used to monitor the shape and size of the cell. The results showed that the Rh123 fluorescence signal in a single-cell increased dramatically over its same-cell control due to the competitive inhibition of paclitaxel and the non-competitive inhibition subjected by CsA.

Research Area(s)

  • Fluorescence measurement, MDA-MB-231 cell, Microfluidic chip, Multidrug resistance, Single-cell analysis, Triple-negative breast cancer

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to lbscholars@cityu.edu.hk.

Citation Format(s)

The microfluidic capture of single breast cancer cells for multi-drug resistance assays. / Parekh, Karan; Sharifi, Hamide; Khamenehfar, Avid; Beischlag, Timothy V.; Payer, Robert T.M.; Li, Paul C.H.

Methods in Enzymology. Vol. 628 Academic Press Inc., 2019. p. 113-127 (Methods in Enzymology; Vol. 628).

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)12_Chapter in an edited book (Author)peer-review