The Endometrial Microbiota—16S rRNA Gene Sequence Signatures in Healthy, Pregnant and Endometritis Dairy Cows

Anne A. M. J. Becker; Stacie Munden; Evonne McCabe; Daniel Hurley; Séamus Fanning; Aspinas Chapwanya; Patrick Butaye

doi:10.3390/vetsci10030215

The Endometrial Microbiota—16S rRNA Gene Sequence Signatures in Healthy, Pregnant and Endometritis Dairy Cows

Anne A. M. J. Becker, Stacie Munden, Evonne McCabe, Daniel Hurley, Séamus Fanning, Aspinas Chapwanya, Patrick Butaye^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

13 Citations (Scopus)

36 Downloads (CityUHK Scholars)

Abstract

Endometritis is one of the most important causes of infertility in dairy cows, resulting in high economic losses in the dairy industry. Though the presence of a commensal uterine microbiota is now well established, the complex role of these bacteria in genital health, fertility, and susceptibility to uterine diseases remains unclear. In this study, we explore the endometrial microbiota through 16S rRNA gene profiling from cytobrush samples taken ex vivo from healthy, pregnant, and endometritis cows. There were no significant differences between healthy and pregnant cows, whose uterine microbiota were dominated by Streptococcus, Pseudomonas, Fusobacterium, Lactococcus and Bacteroides. Compared to pregnant and clinically healthy cows, the uterine bacterial community of endometritis cows was significantly decreased in species diversity (p < 0.05), reflecting uneven community composition in different patterns with either dominance of Escherichia-Shigella, Histophilus, Bacteroides and Porphyromonas or Actinobacteria. © 2023 by the authors.

Original language	English
Article number	215
Number of pages	13
Journal	Veterinary Sciences
Volume	10
Issue number	3
Online published	10 Mar 2023
DOIs	https://doi.org/10.3390/vetsci10030215
Publication status	Published - Mar 2023
Externally published	Yes

Research Keywords

16S rRNA gene amplicon-based metagenomics
bovine endometritis
post-partum uterine disease
uterine microbiome

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This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/

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title = "The Endometrial Microbiota—16S rRNA Gene Sequence Signatures in Healthy, Pregnant and Endometritis Dairy Cows",

abstract = "Endometritis is one of the most important causes of infertility in dairy cows, resulting in high economic losses in the dairy industry. Though the presence of a commensal uterine microbiota is now well established, the complex role of these bacteria in genital health, fertility, and susceptibility to uterine diseases remains unclear. In this study, we explore the endometrial microbiota through 16S rRNA gene profiling from cytobrush samples taken ex vivo from healthy, pregnant, and endometritis cows. There were no significant differences between healthy and pregnant cows, whose uterine microbiota were dominated by Streptococcus, Pseudomonas, Fusobacterium, Lactococcus and Bacteroides. Compared to pregnant and clinically healthy cows, the uterine bacterial community of endometritis cows was significantly decreased in species diversity (p < 0.05), reflecting uneven community composition in different patterns with either dominance of Escherichia-Shigella, Histophilus, Bacteroides and Porphyromonas or Actinobacteria. {\textcopyright} 2023 by the authors.",

keywords = "16S rRNA gene amplicon-based metagenomics, bovine endometritis, post-partum uterine disease, uterine microbiome",

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T1 - The Endometrial Microbiota—16S rRNA Gene Sequence Signatures in Healthy, Pregnant and Endometritis Dairy Cows

AU - Becker, Anne A. M. J.

AU - Munden, Stacie

AU - McCabe, Evonne

AU - Hurley, Daniel

AU - Fanning, Séamus

AU - Chapwanya, Aspinas

AU - Butaye, Patrick

PY - 2023/3

Y1 - 2023/3

N2 - Endometritis is one of the most important causes of infertility in dairy cows, resulting in high economic losses in the dairy industry. Though the presence of a commensal uterine microbiota is now well established, the complex role of these bacteria in genital health, fertility, and susceptibility to uterine diseases remains unclear. In this study, we explore the endometrial microbiota through 16S rRNA gene profiling from cytobrush samples taken ex vivo from healthy, pregnant, and endometritis cows. There were no significant differences between healthy and pregnant cows, whose uterine microbiota were dominated by Streptococcus, Pseudomonas, Fusobacterium, Lactococcus and Bacteroides. Compared to pregnant and clinically healthy cows, the uterine bacterial community of endometritis cows was significantly decreased in species diversity (p < 0.05), reflecting uneven community composition in different patterns with either dominance of Escherichia-Shigella, Histophilus, Bacteroides and Porphyromonas or Actinobacteria. © 2023 by the authors.

AB - Endometritis is one of the most important causes of infertility in dairy cows, resulting in high economic losses in the dairy industry. Though the presence of a commensal uterine microbiota is now well established, the complex role of these bacteria in genital health, fertility, and susceptibility to uterine diseases remains unclear. In this study, we explore the endometrial microbiota through 16S rRNA gene profiling from cytobrush samples taken ex vivo from healthy, pregnant, and endometritis cows. There were no significant differences between healthy and pregnant cows, whose uterine microbiota were dominated by Streptococcus, Pseudomonas, Fusobacterium, Lactococcus and Bacteroides. Compared to pregnant and clinically healthy cows, the uterine bacterial community of endometritis cows was significantly decreased in species diversity (p < 0.05), reflecting uneven community composition in different patterns with either dominance of Escherichia-Shigella, Histophilus, Bacteroides and Porphyromonas or Actinobacteria. © 2023 by the authors.

KW - 16S rRNA gene amplicon-based metagenomics

KW - bovine endometritis

KW - post-partum uterine disease

KW - uterine microbiome

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U2 - 10.3390/vetsci10030215

DO - 10.3390/vetsci10030215

M3 - RGC 21 - Publication in refereed journal

C2 - 36977254

SN - 2306-7381

VL - 10

JO - Veterinary Sciences

JF - Veterinary Sciences

IS - 3

M1 - 215

ER -

The Endometrial Microbiota—16S rRNA Gene Sequence Signatures in Healthy, Pregnant and Endometritis Dairy Cows

Abstract

Research Keywords

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