TY - JOUR
T1 - The effects of Ce on the proliferation, osteogenic differentiation and mineralization function of MC3T3-E1 cells in vitro
AU - Liu, Dandan
AU - Zhang, Jinchao
AU - Li, Yaping
AU - Wang, Shuxiang
AU - Yang, Mengsu
PY - 2012/11
Y1 - 2012/11
N2 - The effects of Ce on the proliferation, osteogenic differentiation and mineralization function of a murine preos-teoblast cell line MC3T3-E1 in vitro were investigated at cell and molecular levels. The results showed that Ce promoted the proliferation, osteogenic differentiation and mineralization function of MC3T3-E1 cells at concentrations of 0.0001, 0.001, 0.01, 0.1 and 1 μM, but turned to inhibit the proliferation, osteogenic differentiation and mineralization function at concentrations of 10, 100 and 1000 μM. Ce displayed the up-regulation of Runx2, BMP2, ALP, BSP, Col I and OCN genes at concentrations of 0.0001 and 0.1 μM; these genes were down-regulated in the MC3T3-E1 cells treated with 1000 μM Ce. The expression of BMP2, Runx2 and OCN proteins was promoted by Ce at concentrations of 0.0001 and 0.1 μM, but these proteins were down-regulated after 1000 μM Ce treatment. The results suggest that Ce likely up-regulates or down-regulates the expression of Runx2, which subsequently up- or down-regulates OB marker genes Col I and BMP2 at early stages and ALP and OCN at later stages of differentiation, thus causing to promote or inhibit the proliferation, osteogenic differentiation and mineralization function of MC3T3-E1 cells. © Springer Science+Business Media, LLC 2012.
AB - The effects of Ce on the proliferation, osteogenic differentiation and mineralization function of a murine preos-teoblast cell line MC3T3-E1 in vitro were investigated at cell and molecular levels. The results showed that Ce promoted the proliferation, osteogenic differentiation and mineralization function of MC3T3-E1 cells at concentrations of 0.0001, 0.001, 0.01, 0.1 and 1 μM, but turned to inhibit the proliferation, osteogenic differentiation and mineralization function at concentrations of 10, 100 and 1000 μM. Ce displayed the up-regulation of Runx2, BMP2, ALP, BSP, Col I and OCN genes at concentrations of 0.0001 and 0.1 μM; these genes were down-regulated in the MC3T3-E1 cells treated with 1000 μM Ce. The expression of BMP2, Runx2 and OCN proteins was promoted by Ce at concentrations of 0.0001 and 0.1 μM, but these proteins were down-regulated after 1000 μM Ce treatment. The results suggest that Ce likely up-regulates or down-regulates the expression of Runx2, which subsequently up- or down-regulates OB marker genes Col I and BMP2 at early stages and ALP and OCN at later stages of differentiation, thus causing to promote or inhibit the proliferation, osteogenic differentiation and mineralization function of MC3T3-E1 cells. © Springer Science+Business Media, LLC 2012.
KW - Cerium
KW - Mineralization
KW - Osteogenic differentiation
KW - Proliferation
UR - http://www.scopus.com/inward/record.url?scp=84867572409&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84867572409&origin=recordpage
U2 - 10.1007/s12011-012-9423-8
DO - 10.1007/s12011-012-9423-8
M3 - RGC 21 - Publication in refereed journal
C2 - 22528784
SN - 0163-4984
VL - 149
SP - 291
EP - 297
JO - Biological Trace Element Research
JF - Biological Trace Element Research
IS - 2
ER -