The CTX-M-14 plasmid pHK01 encodes novel small RNAs and influences host growth and motility

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Xuan Liu
  • Carmen O. K. Law
  • Ya Wang
  • Wai U Lo
  • Xing Weng
  • Ting Fung Chan
  • P. L. Ho

Related Research Unit(s)

Detail(s)

Original languageEnglish
Article numberfix090
Journal / PublicationFEMS Microbiology Ecology
Volume93
Issue number7
Publication statusPublished - 1 Jul 2017

Abstract

The dissemination of extended-spectrum β-lactamases (ESBLs) genes among bacteria is commonly achieved by plasmid conjugation. In the last decade, the CTX-M type enzyme was the most widespread and prevalent ESBLs in the world. In Hong Kong and mainland China, among the commonly found CTX-M-carrying plasmids were pHK01 and pHK01-like plasmids, which belong to incompatibility group FII (IncFII). In this work, we studied the physiological effect caused by the pHK01 plasmid in bacterial host Escherichia coli J53. The plasmid did not affect cell growth of the host but reduced their motility. The reduction of host motility was attributed to downregulation of genes that encode the flagellar system. We also identified several plasmid-encoded sRNAs, and showed that the overexpression of one of them, AS-traI, in the presence of pHK01 plasmid shortened the lag phase of host growth. In addition to the study of pHK01 in bacteria, we also developed a fast and incompatibility group-specific curing method using countertranscribed RNA, which could be of general usage for studying plasmid-host interaction in clinical aspects.

Research Area(s)

  • antibiotic resistance plasmid, pHK01, RNA-Seq, motility, sRNA, ESBL-plasmid curing

Citation Format(s)

The CTX-M-14 plasmid pHK01 encodes novel small RNAs and influences host growth and motility. / Jiang, Xinlei; Liu, Xuan; Law, Carmen O. K.; Wang, Ya; Lo, Wai U; Weng, Xing; Chan, Ting Fung; Ho, P. L.; Lau, Terrence C K.

In: FEMS Microbiology Ecology, Vol. 93, No. 7, fix090, 01.07.2017.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal