Temporal changes in Sphingomonas and Mycobacterium populations in mangrove sediments contaminated with different concentrations of polycyclic aromatic hydrocarbons (PAHs)

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Chuling Guo
  • Lin Ke
  • Zhi Dang
  • Nora Fungyee Tam

Detail(s)

Original languageEnglish
Pages (from-to)133-139
Journal / PublicationMarine Pollution Bulletin
Volume62
Issue number1
Publication statusPublished - Jan 2011

Abstract

The change in community diversity and structure of the indigenous, dominant, polycyclic aromatic hydrocarbon (PAH)-degrading bacterial genera, Sphingomonas and Mycobacterium, due to contamination in the environment is not very well known. A combination of PCR-DGGE with specific primers and a cultivation-dependent microbiological method was used to detect different populations of Sphingomonas and Mycobacterium in mangrove sediments. The structure of the entire bacterial community (including Sphingomonas) did not show a shift due to environmental contamination, whereas the diversity of Mycobacterium populations in mangrove sediments with higher PAH contamination increased from exposure between Day 0 and Day 30. The isolated Mycobacterium strains migrated to the same position as the major bands of the bacterial communities in Mycobacterium-specific DGGE. A dioxygenase gene system, nidA, which is commonly found in PAH-degrading Mycobacterium strains, was also detected in the more highly contaminated sediment slurries. The present study revealed that Mycobacterium species were the dominant PAH-degraders and played an important role in degrading PAHs in contaminated mangrove sediments. © 2010 Elsevier Ltd.

Research Area(s)

  • Mangrove sediment, Microbial community structure, Mycobacterium, PAH-degrading bacteria, PAHs, Sphingomonas

Citation Format(s)

Temporal changes in Sphingomonas and Mycobacterium populations in mangrove sediments contaminated with different concentrations of polycyclic aromatic hydrocarbons (PAHs). / Guo, Chuling; Ke, Lin; Dang, Zhi; Tam, Nora Fungyee.

In: Marine Pollution Bulletin, Vol. 62, No. 1, 01.2011, p. 133-139.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review